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6 risultati
Coxiella burnetii antigens for detection of Q fever antibodies by ELISA in human sera.
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Different concentrations of chemically-treated (by potassium periodate oxidation or mild acid hydrolysis) purified phase I Coxiella burnetii (C.b.) corpuscles and natural (untreated) purified phase I and phase II C.b. corpuscles were compared by ELISA for detection of both phase I (directed to
In vivo endogenous spore formation by Coxiella burnetii in Q fever endocarditis.
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OBJECTIVE
To determine whether Coxiella burnetii, the aetiological agent of Q fever, undergoes endogenous spore-like formation, the crucial stage of the developmental cycle, in the infected cardiac valves of patients with chronic Q fever endocarditis.
METHODS
Surgically removed valves from three
[Solid-phase immunoenzyme analysis (ELISA)--a test for diagnosing Q fever (comparison with the complement fixation and immunofluorescence methods)].
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Purified phase I Coxiella burnetii corpuscles treated chemically in different ways (by potassium periodate, mild acid hydrolysis or trichloroacetic acid) were compared by ELISA for their ability to detect phase II (directed to antigen 2) and phase I (directed to antigen 1) antibodies in sera from
Suitability of various Coxiella burnetii antigen preparations for detection of serum antibodies by various tests.
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Comparison of four serological tests (complement fixation (CF) test, microagglutination (MA) test, microimmunofluorescence (MIF) test, and enzyme-linked immunosorbent assay (ELISA)) for detection of post-infection antibody response in human and animal sera revealed a low sensitivity of the CF test
Sustained axenic metabolic activity by the obligate intracellular bacterium Coxiella burnetii.
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Growth of Coxiella burnetii, the agent of Q fever, is strictly limited to colonization of a viable eukaryotic host cell. Following infection, the pathogen replicates exclusively in an acidified (pH 4.5 to 5) phagolysosome-like parasitophorous vacuole. Axenic (host cell free) buffers have been
Evidence of Coxiella burnetii in Punjab province, Pakistan.
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Coxiella burnetii causes query (Q) fever, an important zoonotic disease with worldwide significance. The role of environment in the ecology of C. burnetti, and its influence on seroconversion in animals has not been elucidated in Pakistan. We carried out a cross-sectional study in Punjab province to
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