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shwartzman phenomenon/carbohydrate
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The Shwartzman phenomenon in equine species.
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The occurrence of the Local Shwartzman Reaction (LSR) in equine species has not previously been reported. The molecular mechanism appears identical to that reported for the rabbit and other species. The immunopathologic and histopathologic similarities of the experimentally induced LSR in horses and
Characterization of gonococcal antigens responsible for induction of bactericidal antibody in disseminated infection.
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The role of gonococcal antigens in serum bactericidal activity was determined for an isolate of Neisseria gonorrhoeae from a patient with disseminated gonococcal infection (DGI). Gonococcal outer membranes were purified by differential ultracentrifugation of sheared organisms treated with EDTA. The
Isolation of a serogroup 1-specific antigen from Legionella pneumophila.
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Outer membrane material was extracted from a serogroup 1 strain of Legionella pneumophila using ethylenediaminetetraacetate. Ferritin-conjugated antiserum reacted only at the surface of the organism, as seen by electron microscopy. Outer membrane material was fractionated into five peaks by
Characterization of endotoxin from the rumen bacterium Megasphaera elsdenii.
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Phenol-water extraction of Megasphaera elsdenii, a predominant gram-negative coccus in rumens of cattle fed high-grain diets, yielded material that exhibited typical characteristics of endotoxin. The extract was lethal to mice and to chicken embryos, caused biphasic fever in rabbits, leukopenia in
Cell wall lipopolysaccharides from xanthomonas species.
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Volk, Wesley A. (University of Virginia, Charlottesville). Cell wall lipopolysaccharides from Xanthomonas species. J. Bacteriol. 91:39-42. 1966.-The lipopolysaccharides from 20 species of Xanthomonas were extracted and purified. Biological studies suggest that these lipopolysaccharides are analogous
Characterization of lipopolysaccharide of Haemophilus influenzae.
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Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and
Studies on endotoxin of Erwinia herbicola and their biological activity.
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The endotoxin (lipopolysaccharide) preparations were extracted by the BOIVIN method from 10 strains of Erwinia herbicola isolated from the air of grain mills and from human and animal sources. It was found in assays for biological activity that these preparations had true endotoxic properties:
Enzymatically deacylated Neisseria lipopolysaccharide (LPS) inhibits murine splenocyte mitogenesis induced by LPS.
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Acyloxyacyl hydrolase is a leukocyte enzyme that selectively removes the secondary acyl chains from the lipid A moiety of gram-negative bacterial lipopolysaccharides (LPS). As predicted by the reported contribution of secondary acyl chains to the bioactivities of lipid A analogs, enzymatic
Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27.
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The lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans strains Y4 and N27 was isolated by the phenol-water procedure. Morphologically, the molecule consisted of ribbon and branched filaments which comprised 3% of the cellular dry weight. Chemical analysis of the isolated and purified
Biological and chemical characterization of lipopolysaccharide from Selenomonas spp. in human periodontal pockets.
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Pure lipopolysaccharide extracted from Selenomonas spp. isolated from human periodontal pockets was composed of 23.7% carbohydrate, 16.5% hexosamine, 1.2% 2-keto-3-deoxyoctonate, 0.7% heptose, 26.0% lipid, 1.8% protein, and 1.3% phosphorus. It was shown to be quite lethal, to have very active
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