trichosanthin/reazioni di ipersensibilità

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Properties of bromodextran-trichosanthin: a comparison with trichosanthin, an anti-AIDS protein.

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Trichosanthin was coupled with bromodextran and the reaction mixture chromatographed on Sephadex G-75 fine to yield two peaks. The first peak was judged to be bromodextran-trichosanthin by SDS-polyacrylamide gel electrophoresis. The yield was optimal when 4% trichosanthin and 8% bromodextran T20

[The physical properties, pharmacology and antifertility action of crystal trichosanthin protein liposome].

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The crystal trichosanthin protein liposome (TPL) was prepared by an emulsion-forming method. The rate of entrapment was 33.0-44.8% and the rate of recovery was 95.15-99.58%. The determination of trichosanthin protein was accomplished by spectrophotometry at 650 nm, thus eliminating completely the

[Intra-amniotic injection of crystal trichosanthin for induction of labour in second trimester pregnancy].

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Labor was induced in a group of 200 women in 2nd trimester of pregnancy by crystal trichosanthia injected into the amniotic cavity. It was effective and the success rate was 99.5%. The average abortion inducing interval was 4.69 days porm 1.15 days. Blood loss was scanty during labor in 90.5% cases,

Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand.

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BACKGROUND Trichosanthin (TCS) induces a type 2 helper T lymphocyte (T(H)2) immune response that leads to the production of TCS-specific immunoglobulin (Ig) E and a subsequent allergic reaction in vivo. However, events immediately following treatment with TCS are poorly understood. OBJECTIVE We

Lowering of trichosanthin immunogenicity by site-specific coupling to dextran.

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Trichosanthin is a type I ribosome-inactivating protein possessing a broad spectrum of biological and pharmacological activities. Therapeutic use of this compound is hampered by its immunogenicity. It was shown earlier that coupling of dextran to trichosanthin can increase plasma half-life and

Trichosanthin treatment of HIV-induced immune dysregulation.

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Twenty patients with the acquired immunodeficiency syndrome (AIDS), AIDS-related complex (ARC) or asymptomatic HIV infection (HIV+) were given 20 mcg kg-3 trichosanthin (TCS; 'Compound Q'), a ribosome-inactivating protein with in vitro antiviral activity against human immunodeficiency virus (HIV)

Role of blood transfusion in trichosanthin-induced anaphylaxis.

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Trichosanthin (TCS) is a type 1 ribosome inactivating protein extracted from Chinese medicinal herb. It possesses various biological functions such as abortifacient, anti-tumor and anti-viral activities. Clinical trial of this compound against human immunodeficiency virus (HIV) had been conducted.

Novel IgE peptide-based vaccine prevents the increase of IgE and down-regulates elevated IgE in rodents.

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BACKGROUND Immunotherapy with anti-IgE antibodies for treatment of allergy is promising but a short half-life and extremely high cost limit its application. OBJECTIVE We sought to develop IgE vaccines that induce longer-lasting auto-antibodies to neutralize self-IgE as an alternative

Construction and diversity analysis of a murine IgE phage surface display library.

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To make further investigation of the IgE antibody repertoire in Trichosanthin (TCS) allergic responses, a murine IgE phage surface display library was constructed (3.0 x 10(5) independent clones). We first constructed the V epsilon cDNA library (4.6 x 10(5) independent clones) and V kappa cDNA

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