[Anti-leukemia effect of sophora flavescens combined with the low molecular weight natural tumor suppressor of the human fetal liver and its mechanism].

OBJECTIVE

To explore the effect of sophora flavescens (SF) combined low molecular weight natural tumor suppressor(LMW-NTS) on normal human bone marrow CFU-GM and acute myelogenous leukemia cells and its mechanism.

METHODS

CFU-GM colony culture, cell culture, Wrigh-Giemsa stain, 3H-TdR incorporation test, agarose gel electrophoresis of DNA fragment and RT-PCR were employed in this research.

RESULTS

The combination of SF and LMW-NTS greatly inhibited the proliferation of acute meylogenous leukemia cells, induced their apoptosis, and down-regulated the c-Myc expression as compared with SF or LMW-NTS alone.

CONCLUSIONS

The anti-leukemia effect of the combination of SF and LMW-NTS is superior to SF or LMW-NTS alone.