[Effects of 5F from Pteri semipinnata on the expression of ETS-1 mRNA and VEGF protein of highly metastatic ovarian carcinoma HO-8910PM cells].

OBJECTIVE

To study the effect of 5F from Pteri semipinnata L. on the expression of ETS-1 mRNA and VEGF protein, and to investigate the antitumor mechanisms of 5F from Pteri semipinnata L.

METHODS

RT-PCR assay was used to assess the expression level of ETS-1 mRNA. The expression level of VEGF protein was assessed by Western blot analysis.

RESULTS

The expression level of of VEGF protein obviously decreased in HO-8910PM cells treated with 25 to approximately 100 micromol/L 5F for 24 hours. 5F significantly down-regulated the expression of ETS-1 mRNA.

CONCLUSIONS

The antitumor activity of 5F from Pteri semipinnata L is involved in the expression of ETS-1 mRNA and VEGF protein.