Botanical Studies 2019-Jul
Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia.
רק משתמשים רשומים יכולים לתרגם מאמרים
התחבר הרשם
הקישור נשמר בלוח
מילות מפתח
תַקצִיר
RESULTS
The organogenic cultures obtained from inflorescence explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) under a light intensity of 10 μmol m-2 s-1 or 45 μmol m-2 s-1. The highest callus proliferation index (93.15%) with 1.0 mg L-1 BA + 0.1 mg L-1 NAA under a light intensity of 10 μmol m-2 s-1. The best shoot proliferation rates were on media with either 1 mg L-1 BA + 0-0.4 mg L-1 NAA (65.57-81.01%) under a light intensity of 45 μmol m-2 s-1. The highest root length (15.57 mm) and the highest rooting frequency (17 roots per shoot) were obtained when adventitious shoots were inoculated on MS medium with 0.4 mg L-1 NAA + 0.4 mg L-1 IBA. The survival rate of the transplanted plantlets was about 100%. The efficient micropropagation protocol proliferated Haworthia regenerate plants from inflorescence within 11 weeks.