Hydrogen sulfide directs metabolic flux towards the lignan biosynthesis in Linum album hairy roots.

Hydrogen sulfide (H₂S) has been recently found as an important signaling molecule especially in root system architecture of plants. The regulation of root formation through H₂S has been reported in previous works; while the profiling of metabolites in response to H₂S is not clearly discussed. To this end, different concentrations of sodium hydrosulfide (an H₂S donor) were applied to the culture of Linum album hairy roots. Subsequently, the amino acid profiles, soluble carbohydrates, and central intermediates of phenylpropanoid pathway with two branches of lignans and flavonoids were assessed by spectroscopy and high performance liquid chromatography techniques. An analysis of the signaling molecules (nitric oxide, hydrogen peroxide, and salicylic acid) was also conducted as they proposed to act in conjunction with H₂S. The H₂S activated antioxidant systems and caused a shift from flavonoid to lignan production (podophyllotoxin and 6-methoxypodophyllotoxin); although, some of the flavonoids increased in a dose-dependent manner. The H₂S decreased the contents of phenylalanine and tyrosine as substrates of the phenylpropanoid pathway, but increased proline and histidine as an osmolyte and antioxidant, respectively. These findings propose that H₂S modulates other signaling molecules, regulates free amino acids, and mediates biosynthesis of lignans and flavonoids in the phenylpropanoids biosynthesis pathway.