[Simultaneous determination of panaxynol and panaxydol in fibrous root of Panax ginseng by HPLC].

OBJECTIVE

To establish an HPLC method for simultaneous determination of panaxynol and panaxydol from the fibrous root of Panax ginseng.

METHODS

The analysis was performed on Elite C18 column (4.6 mm x 150 mm, 5 microm) with mobile phase gradient of CH3CN-water at a flow rate of 1.0 mL x min(-1). The detection wavelength was 230 nm, and the detection temperature was ambient.

RESULTS

The linear range were 0.70-3.50 microg (r = 0.9995) for panaxynol, and 0.64-3.20 microg (r = 0.9999) for panaxydol. The average recoveries were 99.1% (RSD 1.7%) and 99.3% (RSD 1.2%), respectively.

CONCLUSIONS

The HPLC method is simple, rapid and reproducible, which can be used for the quality control of the fibrous root of P. ginseng.