[Studies on transplanting suspension-cultured protocorms of Dendrobium candidum onto solid culture medium].

OBJECTIVE

The suspension-cultured protocorms of Dendrobium candidum were transplanted on the solid culture medium for studying the factors influencing their differentiation and growth.

METHODS

The growth and differentiation of protocorms were detected when different base-media, plant phytohormones, carbohydrates and pH were applied.

CONCLUSIONS

The diluted MS medium promotes the growth of protocorms but inhibit differentiation. The phytohormone NAA, IAA, IBA and GA are helpful to the growth and differentiation of protocorms, however, the low pH values and presence of autoclaved fungi have suppressive effects on protocorms. The fungi also induce morphological variation of protocorms.