AM fungi mediated bioaccumulation of hexavalent chromium in Brachiaria mutica-a mycorrhizal phytoremediation approach

The experiment was designed to evaluate the roles of Rhizophagus irregularis on chlorophyll fluorescence and chromium bioaccumulation in a grass species (Brachiaria mutica) by supplementing Cr⁺⁶ at different concentrations. Arbuscular Mycorrhizal Fungi (AMF) association facilitated lessening of chromium level in contaminated soil and enhanced chromium bioavailability in Brachiaria mutica. The mycorrhizal inoculated increased the chlorophyll (0.925 mg/g), carotenoid (0.127 mg/g), protein (2.883 mg/g), proline (0.889 mg/g) contents and activities of antioxidant enzymes like catalase, ascorbate peroxidase and glutathione peroxidase. The mycorrhizal inoculated plants also showed enhanced overall photosynthetic performance (PI_ϕ = 2.473) and enhanced PS-II to PS-I electron transport as evident from yield parameter (0.712) and TR₀/RC (2.419) for 60 mg/kg Cr⁺⁶ treatment. The observations suggest that AMF association could defend the plants from chromium stress by elevating the number of antioxidants in plants. Rhizophagus irregularis was found to maintain a successful symbiotic relationship with Brachiaria mutica in chromium contaminated soil. The observations recommended that Rhizophagus irregularis in association with Brachiaria mutica would be an innovative approach for decontamination of Cr⁺⁶.

Keywords: AMF; Brachiaria mutica; Chlorophyll fluorescence; Decontamination; Hexavalent chromium.