Modified citrus pectins by UV/H ₂ O ₂ oxidation at acidic and basic conditions: Structures and in vitro anti-inflammatory, anti-proliferative activities

Two modified citrus pectins, MCP4 and MCP10, were prepared by UV/H₂O₂ treatment at pH 4 and pH 10, respectively, and their structures were characterized. MCP10 had a rhamnogalacturonan-I (RG-I) enriched backbone with a high degree of branching (DB ∼61 %) and a low methoxylation degree (24 %). MCP4 had a homogalacturonan enriched backbone with a high degree (46 %) of methoxylation and a low DB (∼41 %) of RG-I branches. MCP10 exhibited a higher anti-inflammatory activity than MCP4 in suppressing the NF-κB expression and the production of pro-inflammatory factors TNF-α and IL-1β of THP-1 cells stimulated by lipopolysaccharide. MCP10 also showed a stronger inhibitory effect on Caco-2 cell proliferation. The stronger bioactivities of MCP10 may be attributable to the abundant branches and the proper length of terminal galactan residues attached to the RG-I domain.

Keywords: Alkaline and acidic pH; Anti-inflammation; Anti-proliferation; Modified citrus pectin; UV/H(2)O(2) oxidation.