Kaempferia marginata Carey (Zingiberaceae family) has been traditionally used in the treatment of inflammation. The whole plant decoction is used for treatment of fever. Ethanol extracts which exert potent anti-inflammatory properties are selected for wound healing assay.This study aimed to investigate activities of the extract and gel formulation on anti-inflammatory and wound healing activities. The anti-inflammatory and wound healing properties from this plant could support its traditional uses and obtain a new pharmaceutical product with good physical, chemical and biological stabilities.The anti-inflammatory activity was tested on anti-nitric oxide (NO) production using RAW264.7 cells and wound healing assay using human dermal fibroblast (HDF) cells.
RESULTS
The results found that the anti-inflammatory activity of gel containing K. marginata at 10% w/w showed the highest activity with an IC
50 value of 12.50 μg/ml (Diclofenac gel, IC
50 value = 64.90 μg/ml). It also revealed that wound healing activities of K. marginata gel (5% w/w) showed the highest % cell viability (134.05%) and the highest % cell migration at 85.20 using HDF cells.
The present study shows that gel containing K. marginata extracts have good anti-inflammatory and wound healing properties in vitro.