[Genetic polymorphism of glutathione S-transferase T1 associated with idiopathic azoospermia and oligospermia].
キーワード
概要
OBJECTIVE
To investigate the association of glutathioneS-transferase T (GSTT1) gene polymorphism with azoospermia and oligospermia.
METHODS
Semen samples from 34 patients with idiopathic azoospermia, 40 patients with idiopathic oligospermia and 53 healthy controls with normal sperm concentration and motility were assessed according to the standards of WHO. The GSTT1 genotypes were identified by multiplex polymerase chain reaction (PCR) with peripheral blood DNA samples.
RESULTS
The frequencies of null GSTTI genotypes in the patients with idiopathic azoospermia, idiopathic oligospermia and the healthy controls were 76.5%, 72.5% and 49.1%, respectively. There was a significant association between the null alleles of GSTT1 and idiopathic azoospermia (odds ratio 3.13, 95% CI 1.20-8.16, P = 0.020) and idiopathic oligospermia (odds radio 2.53, 95% CI 1.06-6.11, P = 0.038).
CONCLUSIONS
The null alleles of GSTTI are a strong predisposing risk factor for idiopathic azoospermia and oligospermia.
