A mouse model in which diabetes mellitus was induced by low-dose streptozotocin (STZ) injection combined with a high fat diet was used to study the effect of two water cress (Lepidium savitum) preparations. Diabetic mice were treated with dried cress powder or with water-soluble extracts (tested at two doses), together with proper control groups. The mice were evaluated after 4 weeks of continuous intervention for type 2 diabetic and associated markers. We determined blood glucose, body weight, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), serum insulin levels, and DNA integrity of hepatic cells. The concentrations of malondialdehyde (MDA) and lipid peroxide (LPO) and the activities of four enzymes that are part of the antioxidant defense system were determined in liver samples, as well as gene expression (by semi-quantitative RT-PCR) and enzyme activity of IRS-1, IRS-2, PI3K, AKT-2 and GLUT4.After 4 weeks of intervention, the levels of TC, TG and LDL-C were significantly (P<0.5) decreased, while HDL-C was significantly increased. Enzyme activity of liver SOD, GSH, GSH-PX and CAT was significantly increased, while concentrations of MDA and LPO were significantly reduced. The transcription level of the 5 assessed genes was increased, with corresponding increases in protein expression.Oral uptake of garden cress can significantly reduce the blood glucose and improve the blood lipid metabolism of diabetic mice. Considerable improvements in the activity of antioxidant defense enzymes were observed in type 2 diabetic mice that improved the body's antioxidant emergency response. This article is protected by copyright. All rights reserved.
