Molluscicidal activity of fatty acids in the kernel of Chimonanthus praecox cv. Luteus against the golden apple snail Pomacea canaliculata

The fatty acid composition of the kernel of Chimonanthus praecox cv. Luteus (FKC) was analyzed by gas chromatography-mass spectrometry (GC-MS), its ability to kill Pomacea canaliculata was detected, and the degree of damage and physiological and biochemical effects of an FKC soaking treatment on the hepatopancreas tissue of P. canaliculata were evaluated. In total, 16 fatty acids were detected in FKC, among which 13 were qualitatively identified; octadecadienoic acid (56.76%) and palmitic acid (17.03%) had the highest contents. After 48 h of treatment with FKC, the hepatopancreas of P. canaliculata had a large area of necrosis. The contents of soluble sugar, soluble protein, and albumin (Alb) in the hepatopancreas of P. canaliculata decreased with increasing FKC concentration. The content of malondialdehyde (MDA) and the activities of cereal third transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (AKP), and acetylcholinesterase (AChE) increased with increasing FKC concentration. The results showed that FKC has an obvious negative effect on the hepatopancreas cell structure and physiological function of P. canaliculata, i.e., has strong molluscicidal activity.

Keywords: Chimonanthus praecox cv. Luteus; Fatty acids; Gas chromatography-mass spectrometry analysis; Hepatopancreas; Molluscicidal activity; Pomacea canaliculata.