Two Zn(II)-based Coordination Polymers: Fe ³⁺ Ion and Prevention Activity Detection On Postpartum Hemorrhage By Regulating Anticoagulant Factor Activity

Two new Zn(II)-based coordination polymers {[Zn₃(L₁)₆(H₂O)]∙(H₂O)₄}_n (1, HL₁ = 4-(tetrazol-5-yl)phenyl-4,2':6',4″-terpyridine) and [Zn₂Cl₂(L₂)₂H₂O]_n (2, HL₂ = 4-([2,2':6',2″'-terpyridin]-4'-yl)benzoic acid) have been successfully prepared using two similar organic ligands with distinct donor groups under similar reaction conditions. The distinct structural features and donor atoms make the two complexes show different water stability, and the complex 1 with good water stability, which can be utilized as the sensor for Fe³⁺ ion detection in water. The value of Stern-Volmer quenching constant of 1 to the Fe³⁺ is 5.77 × 10⁴ M^{- 1}, which lies in the top region of the reported CP-based sensors. The mechanism investigation reveals that the energy transfer of resonance from the complex 1 to the Fe³⁺ ion can account for its fluorescent quenching behavior. The treatment activity of compounds 1 and 2 on the postpartum hemorrhage (PPH) was assessed. First, the cytotoxicity of compounds 1 and 2 on human umbilical vein endothelial cells was assessed with Cell Counting Kit-8 detection kit. Then, to evaluate the prevention of compounds 1 and 2 on the PPH, we conducted the Lowry method and detected the clotting factor IX and anticoagulant factor III contents after the indicated treatment. Finally, the inflammatory response in mice was determined by ELISA method, and the IL-6 and IL-8 levels were determined.

Keywords: Coordination polymer; ELISA assay; Fe3+ sensor; postpartum hemorrhage.