[Proteinases in the muscles of embryos and hens and and in transplantable muscle sarcoma].
키워드
요약
The authors examined the activity of acid proteinases in extracts, obtained by 0,05 M KCl from thigh muscles of 12-and 18-day hen embryos and of a 3-year hen and from muscle sarcoma 12--14 days after its transplantation on thigh muscles of a hen. The activity of the fractions obtained from extracts by gel chromatography on sefadex G-200, was also determined. The highiest activity per unit of protein was established in extracts of embryonal muscles, but the lowest -in cultures of hens. Sarcoma took medean position. Seven fraction of acid proteinases were found in the muscles and in the sarcoma. In most cases sarcoma took mean position among embryonal muscles and muscles of hens in percentage ratios of these fractions. The highiest mollecular (the earliest flowing) acid proteinase fractions in the extract of hen muscles had insignificant activity, but in extracts of sarcoma they had almost 20% of the total activity.