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l ornithine/hypoxia

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Effects of propranolol on rat myocardial l-ornithine decarboxylase activity during exercise and hypoxia.

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Ornithine decarboxylase activity was higher in the left ventricle than in the right ventricle of the rat. Swimming exercise for 2 hours caused an increase in left ventricular but not right ventricular enzyme activity. This effect was not influenced by the prior injection of propranolol, which also

Role of endothelial nitric oxide synthase in hypoxia-induced pial artery dilation.

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Nitric oxide (NO) contributes to hypoxia-induced pial artery dilation, at least in part, through the formation of cGMP and the subsequent release of methionine enkephalin and leucine enkephalin in the newborn pig. In separate studies, these opioids also were observed to elicit NO-dependent pial

Acute hypoxia increases ornithine decarboxylase activity and polyamine concentrations in fetal rat brain.

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The cellular responses to hypoxia are poorly understood. To test the hypothesis that ornithine decarboxylase (ODC; L-ornithine carboxy-lyase; EC 4.1.1.17) activity and polyamine concentrations change in response to acute hypoxia, we performed the following studies. Pregnant Sprague-Dawley rats

The effect of hypoxia and laminin-rich substrates on the proliferative behavior of human neural stem cells.

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Human neural stem cells (hNSCs) possess an enormous potential to be utilized in novel cell-replacement therapies for neurodegenerative diseases and injuries. The hNSCs are a renewable source of cells with the capacity to generate the major cell types of the central nervous system (CNS). However, the

Hypoxia promotes human pulmonary artery smooth muscle cell proliferation through induction of arginase.

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Vascular remodeling and smooth muscle cell proliferation are hallmark pathogenic features of pulmonary artery hypertension (PAH). Alterations in the metabolism of l-arginine via arginase and nitric oxide synthase play a critical role in the endothelial dysfunction seen in PAH. l-arginine metabolism

L-NIO as a novel mechanism for inducing focal cerebral ischemia in the adult rat brain.

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BACKGROUND Ischemic stroke is the most frequent cause of persistent neurological disability in Western societies. New treatment strategies are required and effective in vivo models are crucial to their development. METHODS The current study establishes a novel in vivo rat model of focal striatal

Vasoactivity of nitrite in the iliac artery of the toad, Rhinella marina.

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Nitrite ([Formula: see text]) causes vasodilation in mammals due to the formation of (nitric oxide) NO by endogenous [Formula: see text] reduction in the vascular wall. In this study, we determined if a similar mechanism operates in amphibians. Dual-wire myography of the iliac artery from Rhinella

L-arginine restores endothelium-dependent relaxation in pulmonary circulation of chronically hypoxic rats.

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We investigated whether loss of endothelial-derived relaxing factor (EDRF) activity in the pulmonary vessels of chronically hypoxic rats could be restored by pretreatment with L-arginine. We measured vasodilation to acetylcholine (ACh), calcium ionophore A23187, or linsidomine (Sin-1) under
The ability of the developing myocardium to tolerate oxidative stress during early gestation is an important issue with regard to possible detrimental consequences for the fetus. In the embryonic heart, antioxidant defences are low, whereas glycolytic flux is high. The pro- and antioxidant
Severe hypoxia caused by a lack of vascular supply and an inability to retrieve encapsulated islets transplanted in the peritoneal cavity for biopsy and subsequent evaluation are obstacles to clinical application of encapsulation strategies for islet transplantation. We recently proposed an omentum

Ketoprofen controlled release from composite microcapsules for cell encapsulation: effect on post-transplant acute inflammation.

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Cell encapsulation technology raises hopes in medicine and biotechnology. Encapsulated pancreatic islets is a promising approach for the final solution of Type 1 diabetes. Unfortunately, evidence of long-term encapsulated islet graft survival and functional competence lies behind expectancy. Failure

Arginase II is a target of miR-17-5p and regulates miR-17-5p expression in human pulmonary artery smooth muscle cells.

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Vascular remodeling and smooth muscle cell proliferation are hallmark pathogenic features of pulmonary artery hypertension. MicroRNAs, endogenously expressed small noncoding RNAs, regulate gene expression at the posttranscriptional level. It has previously been shown that miR-17 overexpression in

The role of polyamines in protein-dependent hypoxic tolerance of Drosophila.

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BACKGROUND Chronic hypoxia is a major component of ischemic diseases such as stroke or myocardial infarction. Drosophila is more tolerant to hypoxia than most mammalian species. It is considered as a useful model organism to identify new mechanisms of hypoxic tolerance. The hypoxic tolerance of

Hypoxic Proliferation of Osteosarcoma Cells Depends on Arginase II.

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OBJECTIVE Despite significant advancements in the diagnosis and treatment of osteosarcoma, the overall survival has remained relatively unchanged for over two decades. Hypoxic conditions have been demonstrated in solid tumors and are associated with increased cell proliferation and angiogenesis.

Preparation and in vitro and in vivo characterization of composite microcapsules for cell encapsulation.

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Cell encapsulation technology raises great hopes in medicine and biotechnology. Transplantation of encapsulated pancreatic islets represents a promising approach to the final cure of type 1 diabetes mellitus. Unfortunately, long-term graft survival and functional competence remain only partially
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