A recombinant vector which can transform potato plants so as to confer upon the potato plant immunity against potato virus Y necrosis line is disclosed. The recombinant vector according to the present invention comprises a promoter which functions in a potato plant cell; an operably linked leader sequence of RNA4 of cucumber mosaic virus, which is located downstream of the promoter; and an operably linked sequence which encodes a coat protein of potato virus Y necrosis line, which is located downstream of said leader sequence; and the recombinant vector is capable of transforming potato plants.

Japan Tobacco, Inc.

Recombinant vector, method for giving immunity against PVY-T to potato plant, and potato plant having immunity against PVY-T

The invention concerns a method for detecting the PVY virus which consists in a SNP test or an extension reaction specific to mutations corresponding to R/K.sub.400 et D/E.sub.419, the presence of at least one of said mutations inducting the presence of at least one virulent PVY strain capable of causing necrosis in plants of the Solanaceae family, in particular in potatoes.

Institut National de la Recherche Agronomique

Federation Nationale des Producteurs de Plants de Pomes de Terre

Goupement National Interprofessionnel des Semences, Graines et Plants

Methods for detecting isolates of the potato virus (PVY) responsible for necroses

A biological control agent for plants and a method of inoculating the plants with the biological control agent in order protect against plant disease are provided. The treatment method consists of inoculating the plant with a strain of cucumber mosaic virus (CMV), referred to as the KU1 strain, having an associated viral satellite RNA of SEQ ID NO: 1. Particularly, the KU1 strain may be used to protect plants against a particular viral strain of CMV, referred to as the KU2 strain, characterized by the associated viral satellite RNA of SEQ ID NO: 2, which causes tomato necrosis. Additionally, the KU1 strain may be used to protect against potato spindle tuber viroid (PSTV), which causes tomato stunting, fusarium wilt disease in the tomato (caused by Fusarium oxysporum f. sp. Lycopersicae), and leaf spotting disease in the tomato (caused by Alternaria alternate).

potato/necrosis

Recombinant vector, method for giving immunity against PVY-T to potato plant, and potato plant having immunity against PVY-T

Methods for detecting isolates of the potato virus (PVY) responsible for necroses

Biological control agent for plants

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