scrapie/potassium

Conventional electrophysiological intracellular recording techniques were used to test the hypothesis that enhanced calcium entry via voltage-gated calcium channels or the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor-channel complex may be a primary pathological mechanism triggering

High-titered (greater than 10(10) LD50 [50% lethal dose[/g) preparations of scrapie virus-infected hamster brain were subjected to inactivation by various chemicals, autoclaving, and histopathologic processing. Sodium hypochlorite, which reduced infectivity by approximately 4 log LD50/g of brain

Background: RIDASCREEN® Histamine (enzymatic) is an enzymatic test kit for quantification of histamine in fresh fish, canned fish, fish meal, cheese, and wine. Methods: Fish products are extracted with boiling water, while cheese is extracted with water, and the extract is treated with

To elucidate the mechanisms of ultrasonication on the amyloid fibril formation, we quantitatively determined the ultrasonic power using both calorimetry and potassium iodide (KI) oxidation, and under the properly calibrated ultrasonic power, we investigated the ultasonication-induced amyloid

Pathogenic prion protein scrapie (PrP(sc)) may contaminate soils for decades and remain in water in colloidal suspension, providing infection pathways for animals through the inhalation of ingested dust and soil particles, and drinking water. We used molecular dynamics simulations to understand the

There is a limited number of reports regarding detergents and proteases inactivating, degrading, or destabilizing abnormal prion protein (PrPSc). In the present study, the effect of alkaline detergents and proteases on the breakdown of PrPSc in the absence of proteinase K (PK) (degradation) and the