This study investigated tolerance of Australian native grass species Astrebla lappacea, Themeda australis, and Austrostipa scabra and a tree species Acacia harpophylla to different concentrations of arsenic As(V) (13.34-667.36 μM), Cu2+ (0.5-200 μM), Zn2+ (9-500 μM), Mn2+ (8-10240 μM) and Pb2+