Effects of Litchi chinensis fruit isolates on prostaglandin E(2) and nitric oxide production in J774 murine macrophage cells.

BACKGROUND

Litchi chinensis is regarded as one of the 'heating' fruits in China, which causes serious inflammation symptoms to people.

METHODS

In the current study, the effects of isolates of litchi on prostaglandin E(2) (PGE(2)) and nitric oxide (NO) production in J774 murine macrophage cells were investigated.

RESULTS

The AcOEt extract (EAE) of litchi was found effective on stimulating PGE(2) production, and three compounds, benzyl alcohol, hydrobenzoin and 5-hydroxymethyl-2-furfurolaldehyde (5-HMF), were isolated and identified from the EAE. Benzyl alcohol caused markedly increase in PGE(2) and NO production, compared with lipopolysaccharide (LPS) as positive control, and in a dose-dependent manner. Hydrobenzoin and 5-HMF were found in litchi for the first time, and both of them stimulated PGE(2) and NO production moderately in a dose-dependent manner. Besides, regulation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) mRNA expression and NF-κB (p50) activation might be involved in mechanism of the stimulative process.

CONCLUSIONS

The study showed, some short molecular compounds in litchi play inflammatory effects on human.