Integration and Expression of gfp in the plastid of Medicago sativa L.

Here we describe a protocol of alfalfa (Medicago sativa L.) plastid transformation by which gfp, a gene encoding the green fluorescent protein (GFP), is inserted into plastid genome via particle bombardment and homoplastomic plant is obtained. Plastid engineering is likely to make a significant contribution to the genetic improvement of this crop and the production of vaccines and therapeutic proteins.