Ornithine decarboxylase and phosphatase activity can be stimulated by low concentrations of interferon in human breast cancer cell lines.
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The activity of the enzyme ornithine decarboxylase (ODC) is increased in confluent culture of two breast cancer cell lines (T47D and MCF-7) following a change of medium. The increase in activity is maximum at 12 hours and this level is reduced in cells treated with high concentrations of HuIFN-alpha. However, the increased level of ODC activity seen in the first few hours after medium change can be stimulated by low concentrations of interferon (10-100 units per ml). The activity of the acidic and alkaline phosphatase of the above cell lines are affected by HuIFN-alpha similar to ODC. In stimulating levels of ODC and phosphatases, interferon is acting similar to mitogens, and in T47D and MCF-7 cells this stimulatory effect precedes the inhibitory effect more commonly seen in interferon-treated cells.