Shi yan sheng wu xue bao 1999-Dec
[Promotion of transformation frequency of soybean (Glycine max L.) protoplasts using poly-L-ornithine].
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The foreign Bt gene was transferred into protoplasts of soybean using PEG and PLO methods, respectively. The result indicated that the transformation frequency of PLO method was about 0.1% higher than PEG method. The PCR and Southern blotting analysis of the regeneration plants confirmed the integration of foreign gene into the genome of soybean.