The anti-inflammatory properties of Rhododendron molle G. Don leaf extract in LPS-induced RAW 264.7

Rhododendron molle G. Don is a well-known traditional medicine which has been used to treat rheumatic inflammation. In this study, an inflammatory model of lipopolysaccharide (LPS)-stimulated RAW264.7 cells was established to analyze the anti-inflammatory effect and potential mechanism of the methanol extract of R.molle leaves (RLE). The production of NO and the expression of tumor necrosis factor by LPS were detected by Griess reaction and enzyme linked immunosorbent assay (ELISA). The mRNA expression of TNF-α, IL-1β, IL-6, COX-2 and iNOS was measured by qRT-PCR assay. Griess and qRT-PCR showed that the RLE could significantly concentration-dependently inhibit NO production and the expression of many pro-inflammatory cytokines and inflammatory-related enzymes. Scanning electron microscope (SEM) analysis indicated that RLE could inhibit LPS-stimulated RAW264.7 macrophages activation. The protein level of TNF-α and IL-1β were decreased over 50% at 100 µg/ml of RLE, detected by ELISA. These results indicated that RLE had strong anti-inflammatory and immune activity.

Keywords: Immunomodulation; Pro-inflammatory cytokines; RAW 264.7; Rhododendron molle G. Don; anti-inflammatory.