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3 beta galactosidase/vairenis

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Puslapis 1 nuo 38 rezultatus

Expression of beta-galactosidase and beta-xylosidase genes during microspore and pollen development.

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Tobacco (Nicotiana tabacum L.) microspores at the time of mitosis are characterized by the abundant occurrence of 92- and 98-kDa glycoproteins (GP92 and GP98). GP92 is a soluble protein while GP98 is bound to the insoluble microspore fraction. Both glycoproteins were isolated by affinity
A basic glycosylated beta-galactosidase (PsBGAL) has been purified from pea seeds by 910-fold with a specific activity of 77.33 mumoL min(-1) mg(-1) protein. The purified enzyme is an electrophoretically homogeneous protein consisting of a single protein band with an apparent M(r) of 55 kDa, while

Promoter activities of genes encoding β-galactosidases from Arabidopsis a1 subfamily.

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Promoter regions of each of the six AtBGAL gene of the subfamily a1 of Arabidopsis thaliana were used to drive the expression of the β-glucuronidase gene. The pattern of promoters (pAtBGAL) activity was followed by histological staining during plant development. pAtBGAL1, pAtBGAL3 and pAtBGAL4
The aim of this work is to gain insight into the six members of the a1 subfamily of the β-galactosidases (BGAL) from Arabidopsis thaliana. First, the subcellular location of all these six BGAL proteins from a1 subfamily has been established in the cell wall by the construction of transgenic plants

Purification and characterization of a thermostable beta-galactosidase from kidney beans (Phaseolus vulgaris L.) cv. PDR14.

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Using five different steps, beta-Galactosidase has been purified from kidney beans to apparent electrophoretic homogeneity with approximately 90-fold purification with a specific activity of 281 units mg-1 protein. A single band was observed in native PAGE. Activity staining of the native gel with
Seed coat development in Arabidopsis thaliana involves a complex pathway where cells of the outer integument differentiate into a highly specialized cell type after fertilization. One aspect of this developmental process involves the secretion of a large amount of pectinaceous mucilage into the
The precise nature of the developmental modulation of the activity of cell wall hydrolases that breakdown the wall polysaccharides to maintain cellular sugar homeostasis under sugar starvation environment still remains unclear. In this work, the activity of β-galactosidase (EC 3.2.1.23), a
In growing cells, xyloglucan is thought to connect cellulose microfibrils and regulate their separation during wall extension. In Arabidopsis (Arabidopsis thaliana), a significant proportion of xyloglucan side chains contain β-galactose linked to α-xylose at O2. In this work, we identified AtBGAL10

The Arabidopsis At1g45130 and At3g52840 genes encode beta-galactosidases with activity toward cell wall polysaccharides.

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The Arabidopsis genes At1g45130 and At3g52840 encode the beta-galactosidase isozymes Gal-5 and Gal-2 that belong to Glycosyl Hydrolase Family 35 (GH 35). The two enzymes share 60% sequence identity with each other and 38-81% with other plant beta-galactosidases that are reported to be involved in

Comparative characterization of the Arabidopsis subfamily a1 beta-galactosidases.

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The Arabidopsis genome contains 17 predicted beta-galactosidase genes, all of which belong to glycosyl hydrolase (GH) Family 35. These genes have been further grouped into seven subfamilies based on sequence similarity. The largest of these, subfamily a1, consists of six genes, Gal-1 (At3g13750),

Beta galactosidases in Arabidopsis and tomato - a mini review.

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Beta galactosidases (BGALs) are glycosyl hydrolases that remove terminal β-D-galactosyl residues from β-D-galactosides. There are 17 predicted BGAL genes in the genomes of both Arabidopsis (BGAL1-17) and tomato (TBG1-17). All tested BGALs have BGAL activity but their distinct expression profiles and
In Cicer arietinum, as in several plant species, the β-galactosidases are encoded by multigene families, although the role of the different proteins is not completely elucidated. Here, we focus in 2 members of this family, βIII-Gal and βIV-Gal, with high degree of amino acid sequence identity (81%),

The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels

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PIRIN2 (PRN2) was earlie rreported to suppress syringyl (S)-type lignin accumulation of xylem vessels of Arabidopsis (Arabidopsis thaliana). In the present study we report yeast-two hybrid results supporting the interaction of PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has

Expression of the Arabidopsis AtAux2-11 auxin-responsive gene in transgenic plants.

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Five constructions containing deletions of the promoter from an auxin-inducible gene of Arabidopsis thaliana, AtAux2-11, were fused to the coding region of the reporter gene LacZ, which encodes beta-galactosidase, and a polyadenylation 3'-untranslated nopaline synthase sequence from Agrobacterium.
AtFKBP12 is an Arabidopsis cDNA that encodes a protein similar to the mammalian immunophilin, FKBP12. AtFKBP12 was used as 'bait' in a yeast 2-hybrid system to screen for cDNAs in Arabidopsis encoding proteins that bind to FKBP12. Two partial cDNAs were recovered encoding the C-terminus of a protein
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