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Cloning, localization and expression analysis of vacuolar sugar transporters in the CAM plant Ananas comosus (pineapple).

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In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must

Dark/Light modulation of ribulose bisphosphate carboxylase activity in plants from different photosynthetic categories.

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Ribulose bisphosphate carboxylase/oxygenase (RuBPCase) from several plants had substantially greater activity in extracts from lightexposed leaves than dark leaves, even when the extracts were incubated in vitro with saturating HCO(3) (-) and Mg(2+) concentrations. This occurred in Glycine max,

Analysis of pineapple mealybug wilt associated virus -1 and -2 for potential RNA silencing suppressors and pathogenicity factors.

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Higher plants use RNA silencing to defend against viral infections. As a counter defense, plant viruses have evolved proteins that suppress RNA silencing. Mealybug wilt of pineapple (MWP), an important disease of pineapple, has been associated with at least three distinct viruses, Pineapple mealybug

New sources of dietary myosmine uptake from cereals, fruits, vegetables, and milk.

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Myosmine has been regarded as a specific tobacco alkaloid until investigations pointed out that nuts and nut products constitute a significant source of myosmine. In the present study it is shown that the occurrence of myosmine is widespread throughout a large number of plant families. Using a

Simple protoplast isolation system for gene expression and protein interaction studies in pineapple (Ananas comosus L.).

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UNASSIGNED An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. A quick transformation system is also crucial for the functional analysis of genes along with the study of proteins and their interactions in vivo. Presently,

Transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart.

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Two genes encoding polyphenol oxidase (PPO) were isolated from pineapple (Ananas comosus[L.] Merr. cv. Smooth Cayenne). Sequence analyses showed that both contained a single intron and encoded typical chloroplast-localized PPO proteins, the sequences of which corresponded to two pineapple PPO cDNAs,

Genome-Wide Analysis of the YABBY Transcription Factor Family in Pineapple and Functional Identification of AcYABBY4 Involvement in Salt Stress.

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The plant-specific transcription factor gene family, YABBY, belongs to the subfamily of zinc finger protein superfamily and plays an essential regulatory role in lateral organ development. In this study, nine YABBY genes were identified in the pineapple genome. Seven of them were located on

Survey of Current Distribution of Rotylenchulus reniformis in the United States.

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The reniform nematode, Rotylenchulus reniformis, has been reported from all Gulf Coast states, Arkansas, Hawaii, North Carolina, and South Carolina. Experts in 11 states identified the counties or parishes where the nematode is found and provided information regarding associated soil parameters,

Inoculation of Tomato Spotted Wilt Virus into Cotton.

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Tomato spotted wilt virus (TSWV) is an economically important virus of many crops throughout the world. Cotton, Gossypium hirsutum L., has previously been demonstrated to be susceptible to TSWV (1). During the fall of 1996, cotton was assayed as a potential host of TSWV, as it is an important early

Hydrolytic enzymes in the central vacuole of plant cells.

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The hydrolase content of vacuoles isolated from protoplasts of suspension-cultured tobacco cells, of tulip petals, and of pineapple leaves, and the sedimentation behavior of tobacco tonoplasts were studied. Three precautions were found to be important for the analysis of vacuolar hydrolases and of

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