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Puslapis 1 nuo 60 rezultatus
The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain.
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Degeneration is the process whereby Clostridium acetobutylicum ATCC 824 loses the capacity to produce acetone and butanol after repeated vegetative transfers or in continuous culture. Two degenerate mutants (M5 and DG1) of C. acetobutylicum ATCC 824 do not contain the four genes (ctfA, ctfB, adc,
Characterization of the sol operon in butanol-hyperproducing Clostridium saccharoperbutylacetonicum strain N1-4 and its degeneration mechanism.
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The solventogenic sol operon consisting of bld, ctfA, ctfB, and adc was cloned from Clostridium saccharoperbutylacetonicum strain N1-4. These genes share as high as 95-98% similarity with the corresponding sol genes of Clostridium beijerinckii NCIMB 8052. The N1-4 sol gene cluster was transcribed in
Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells.
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Strain degeneration in solventogenic clostridia is a known problem in the technical acetone-butanol fermentation bioprocess, especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared (FT-IR) spectroscopy of the bacterial cells. Degenerative
Characterization of an immobilized cell, trickle bed reactor during long term butanol (ABE) fermentation.
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Acetone-butanol-ethanol (ABE) fermentation was performed continuously in an immobilized cell, trickle bed reactor for 54 days without, degeneration by maintaining the pH above 4.3. Column clogging was minimized by structured packing of immobilization matrix. The reactor contained two serial glass
Cloning of an NADH-dependent butanol dehydrogenase gene from Clostridium acetobutylicum.
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The acetone-butanol fermentation of C. acetobutylicum is characterized by the unique shift from acid to solvent production. The mechanism of the solventogenic switch involves the induction of several enzymes, including NADH-dependent butanol dehydrogenase (BDH) at the onset of solventogenesis. This
Proteomic analysis to elucidate degeneration of Clostridium beijerinckii NCIMB 8052 and role of Ca(2+) in strain recovery from degeneration.
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Degeneration of solventogenic Clostridium strains is one of the major barriers in bio-butanol production. A degenerated Clostridium beijerinckii NCIMB 8052 strain (DG-8052) was obtained without any genetic manipulation. Supplementation of CaCO3 to fermentation medium could partially recover
Olfactory dysfunction in cerebellar ataxia and multiple system atrophy.
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BACKGROUND
Olfactory dysfunction has been reported in Parkinson's (PD) and Alzheimer's disease (AD).
OBJECTIVE
We studied olfactory function in eight patients with multiple system atrophy of cerebellar type (MSA-C), eleven patients with sporadic cerebellar ataxia of unknown etiology and thirteen
Elevation of oleate-activated phospholipase D activity during thymic atrophy.
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Various phospholipases are thought to be associated with the in vitro apoptosis of thymocytes. In the present study, the in vivo phospholipase D (PLD) activity of rat thymus was studied after whole-body X-irradiation or injection of dexamethasone (DEX). Using exogenous [14C]dipalmitoyl
Possible role of polyamines in gyrate atrophy.
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OBJECTIVE
Gyrate atrophy (GA) is marked by hyperornithinemia and lowered ornithine amino transferase (OAT). However there are patients of GA without hyperornithinemia and those with hyperornithinemia without GA. Some cases of GA have been reported to have low lysine. The purpose of the study was to
Experimental investigation of performance and emissions of a VCR diesel engine fuelled with n-butanol diesel blends under varying engine parameters.
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The continuous rise in the cost of fossil fuels as well as in environmental pollution has attracted research in the area of clean alternative fuels for improving the performance and emissions of internal combustion (IC) engines. In the present work, n-butanol is treated as a bio-fuel and
Evidence for preferential solvation in the cyclohexane/n-butanol binary solvent system.
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We report on the rotational diffusion and vibrational population relaxation dynamics of the polycyclic aromatic hydrocarbon (PAH) perylene in a series of cyclohexane/n-butanol binary solvent systems. The molecular scale heterogeneity of this binary system is seen in both types of data. The
History of the acetone-butanol-ethanol fermentation industry in China: development of continuous production technology.
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The acetone-butanol-ethanol (ABE) fermentation industry in China was started in the early 1950s in Shanghai and expanded rapidly thereafter. At its peak, there were about 30 plants all over the country and the total annual production of solvents reached 170,000 tons. This large enterprise was
High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding.
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A continuous acetone-butanol-ethanol (ABE) production system with high cell density obtained by cell-recycling of Clostridium saccharoperbutylacetonicum N1-4 has been studied. In conventional continuous culture of ABE without cell-recycling, the cell concentration was below 5.2 g l(-1) and the
Acetone-butanol-ethanol (ABE) fermentation in an immobilized cell trickle bed reactor.
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Acetone-butanol-ethanol (ABE) fermentation was successfully carried out in an immobilized cell trickle bed reactor. The reactor was composed of two serial columns packed with Clostridium acetobutylicum ATCC 824 entrapped on the surface of natural sponge segments at a cell loading in the range of
Prolonged conversion of n-butyrate to n-butanol with Clostridium saccharoperbutylacetonicum in a two-stage continuous culture with in-situ product removal.
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n-Butanol was produced continuously in a two-stage fermentor system with integrated product removal from a co-feed of n-butyric acid and glucose. Glucose was always required as a source of ATP and electrons for the conversion of n-butyrate to n-butanol and for biomass growth; for the latter it also
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