Euglycemic-hyperinsulinemic clamp before and after 8 weeks supervised exercise training. Before exercise training the participants will also do an "acute exercise" on 1 hour with muscle biopsies taken before and after exercise and then again 4 hours into recovery.

Type2 Diabetes

Insulin Resistance

Due to biological gender differences, our study does not make it possible to make direct comparisons of data between men and women.
If we had chosen female project participants rather than male, we would also have to take into account whether they were pre- or postmenopausal as well as in the former case where they were in the menstrual cycle during the clamp studies.

Acute exercise

Department of Endocrinology, Odense University Hospital

Abnormalities in the Effects of Insulin and Exercise on Glucose- and Lipid Metabolism in Obesity and Type 2 Diabetes

Metabolic and Molecular Abnormalities in Response to Insulin and Exercise in Obesity and Type 2 Diabetes

Determined by euglycemic-hyperinsulinemic clamp before and after 8-weeks exercise training

Measurements of mitochondrial respiration in permeabilized muscle fiber using a high-resolution respirometer

TaqMan gene expression assays for all relevant genes

Protein abundance and phosphorylation of all enzymes of interest will be studied in human skeletal muscle and fat biopsies by Western blotting and quantified by densitometry in arbitrary units.

Studies of genes and enzymes involved in the regulation of lipid droplets and their morphology and interaction with mitochondria in muscle biopsies determined by gene expression, Westen blotting and transmission electron microscopy (immunogold-labelling), all measured in arbitrary units

Determined by quantitative real-time PCR and Western blotting

Determined before and after 8-weeks exercise training by a graded maximal test on a cycle ergometer using indirect calorimetry.

Determined before and after 8-weeks exercise training by a DXA-scan.

Determined by blood tests before and after 8-weeks exercise training

Maria Houborg Petersen

MD, PhD student

Odense University Hospital

Department of Sports Science, University of Southern Denmark

Prostate Cancer

Patients treated for prostate cancer by radical prostatectomy

Visceral adipose tissue specimen

Urology CHRU-TOURS

University Hospital

Uniersity Hospital

Structural Network on Prostate Cancer in the French Greater West Region

Réseau Structurant Sur le Cancer de la Prostate du Grand Ouest

Past dietary intake of essential fatty acids is quantified by measuring their concentration in white adipose tissue

Exposure to persistant organic pollutants (organochlorine chemicals, which are fat-soluble) is quantified by measuring the concentration in white adipose tissue

chloroform/nutukimas

Abnormalities in the Effects of Insulin and Exercise on Glucose- and Lipid Metabolism in Obesity and Type 2 Diabetes

Structural Network on Prostate Cancer in the French Greater West Region

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