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Occupational wood-dust sensitivity from Euonymus europaeus (spindle tree) and investigation of cross reactivity between E.e. wood and Artemisia vulgaris pollen (mugwort).

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A 44-year-old goldsmith suffered from rhinitis and conjunctivitis after having worked with wood dust from Euonymus europaeus (E.e.) for 15 years. The material was used for drying pieces of jewelry. Very strong reactions could be seen after friction test, scratch test and nasal challenge using wood

Melanotic tumours (Blue Naevi) of spinal nerve roots.

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Four cases interpreted as intraspinal blue naevi are reported. The patients were adults females with an age range between 22 and 60 yr. In three there was a single tumour arising from the cervical posterior nerve roots and in the fourth there were multiple tumours arising from the posterior nerve

Glypican-3 is expressed in rhabdomyosarcomas but not adult spindle cell and pleomorphic sarcomas.

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OBJECTIVE Glypican-3 (GPC3) is a membrane-bound heparan sulphate proteoglycan. Glypicans are predominantly expressed during development in cell- and tissue-specific manners and act as co-receptors for many heparin-binding growth factors, playing important roles in cell growth and differentiation.

E pluribus unum: towards a universal mechanism for spindle assembly.

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Recent data have revealed that the mitotic spindle might form by centrosome-independent mechanisms, even in centrosome-containing cells. This suggests that spindle assembly might proceed by a generally conserved acentrosomal mechanism in all higher eukaryotes, regardless of the presence of

What determines whether chromosomes segregate reductionally or equationally in meiosis?

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Normal meiosis consists of a single round of DNA replication followed by two nuclear divisions. In the 1st division the chromosomes segregate reductionally whereas in the 2nd division they segregate equationally (as they do in mitosis). In certain yeast mutants, a single-division meiosis takes

Solitary sclerotic fibroma of skin: a possible link with pleomorphic fibroma with immunophenotypic expression for O13 (CD99) and CD34.

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BACKGROUND Solitary sclerotic fibroma (SF) presents as a well circumscribed dermal nodule, composed of sparse spindle cells with alternating wavy collagen fibers arranged in a storiform pattern. The histogenesis and nature of this histologically distinct lesion are uncertain. Whether this peculiar

Inflammatory myofibroblastic tumors of the head and neck: evaluation of clinicopathologic and prognostic features.

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Head and neck lesions composed of spindle cells evoke a differential diagnosis which includes a host of benign and malignant entities. One of the less common spindle cell lesions in this region is the inflammatory myofibroblastic tumor (IMT). Although IMTs were originally regarded as "pseudotumors",

Best practices in diagnostic immunohistochemistry: spindle cell neoplasms of the gastrointestinal tract.

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BACKGROUND The proper classification of spindle cell neoplasms of the gastrointestinal tract frequently requires the use of immunohistochemistry, as the histologic appearance of these lesions often overlaps. OBJECTIVE To review the antibodies used in the diagnosis of spindle cell neoplasms of the

Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis.

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It is now clear that a centrosome-independent pathway for mitotic spindle assembly exists even in cells that normally possess centrosomes. The question remains, however, whether this pathway only activates when centrosome activity is compromised, or whether it contributes to spindle morphogenesis

Quartet: a Drosophila developmental mutation affecting chromosome separation in mitosis.

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The Drosophila mutation, quartet, affects development at points in the life cycle that require intense mitotic activity. Examination of embryos affected by the maternal effect of quartet has revealed defects that can be attributed to incomplete chromosome separation at mitosis. These defects include

Biphasic parapharyngeal synovial sarcoma: a cytologic and immunocytologic report of a case.

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BACKGROUND Synovial sarcoma is a rare soft tissue sarcoma in the head and neck region and parapharyngeal space. There is no previous cytologic report of synovial sarcoma of parapharynx. The cytologic and immunocytochemical findings of a parapharyngeal biphasic synovial sarcoma together with

Condensation-decondensation of the gamma-tubulin containing material in the absence of a structurally visible organelle during the cell cycle of Physarum plasmodia.

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Genetic evidence has shown the presence of a common spindle pole organiser in Physarum amoebae and plasmodia. But the typical centrosome and mitosis observed in amoebae are replaced in plasmodia by an intranuclear mitosis devoid of any structurally defined organelle. The fate of gamma-tubulin and of

Consequences of non-extrusion of the first polar body and control of the sequential segregation of homologues and chromatids in mammalian oocytes.

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Absence of polar body formation, or premature chromatin condensation (PCC) in human oocytes can cause infertility. We studied in-vitro maturing mouse oocytes in order to identify risk factors for such conditions, and for the precocious segregation of homologues or chromatids. Treatment with the

Somatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes.

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BACKGROUND The aim of our study was to evaluate the possibility of embryonic or somatic cell haploidization after fusion with intact or enucleated immature oocytes which were subsequently cultured in vitro. Embryonic or somatic cell nuclei do not undergo premature chromosome condensation when fused

Nucleo-cytoplasmic interactions in cell hybrids between mouse oocytes, blastomeres and somatic cells.

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With the help of the technique of Sendai virus-mediated cell fusion, hybrid cells were produced between two maturing oocytes, between maturing oocytes or mature secondary oocytes and interphase blastomeres from 2-cell embryos, and between secondary oocytes and follicle cells (FC). In the first case

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