daucus/carbohydrate

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Complementary immunolocalization patterns of cell wall hydroxyproline-rich glycoproteins studied with the use of antibodies directed against different carbohydrate epitopes.

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Antisera raised against the major hydroxyproline-rich glycoprotein (HRGP) in carrot (Daucus carota L.) taproot, extensin-1, and a minor HRGP, extensin-2, were characterized by western blot analysis, enzyme-linked immunosorbent assay, and periodate oxidation and found to be directed against

Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures.

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Wild carrot (Daucus carota L.) cell suspensions were grown on a mineral salt medium supplemented with 10 mmmyoinositol in the presence and absence of 2.25 mum 2,4-dichlorophenoxyacetic acid (2,4-D), and a variety of carbon sources.The data obtained on growth and embryo number in the absence of 2,4-D

Phosphate uptake, transport and transfer by the arbuscular mycorrhizal fungus Glomus intraradices is stimulated by increased carbohydrate availability.

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* The influence of carbohydrate availability to mycorrhizal roots on uptake, metabolism and translocation of phosphate (P) by the fungus was examined in axenic cultures of transformed carrot (Daucus carota) roots in symbiosis with Glomus intraradices. * 14C-labelled carbohydrates, 33P-phosphate and

Nitrogen and carbohydrate storage in biennials originating from habitats of different resource availability.

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Four biennial species (Arctium tomentosum, Cirsium vulgare, Dipsacus sylvester and Daucus carota) which originate from habitats of different nutrient availability were investigated in a 2-year experiment in a twofactorial structured block design varying light (natural daylight versus shading) and

Effects of methyl mercury on arrays of microtubules and macromolecular synthesis in Daucus carota cultures.

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Cell suspension cultures of Daucus carota were exposed to methyl mercury at concentrations between 0 and 6 micrograms/ml for 1, 3, or 24 hr. Microtubule arrays exhibited no detectable disruption (as compared with controls) when treated with 1, 2, and 3 micrograms/ml methyl mercury. Disorganization

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by C NMR.

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The uptake and utilization of sucrose by embryogenic suspension cultures of carrot (Daucus carota L.) growing in the presence of 2,4-D and by somatic embryos derived from these cultures was monitored using (13)C nuclear magnetic resonance. The exogeneously supplied sucrose was completely hydrolyzed

Quantifying biochemical quality parameters in carrots (Daucus carota L.) - FT-Raman spectroscopy as efficient tool for rapid metabolite profiling.

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Application of FT-Raman spectroscopy for simultaneous quantification of carotenoids, carbohydrates, polyacetylenes and phenylpropanoids with high bioactive potential was investigated in storage roots of Daucus carota. Within single FT-Raman experiment carbohydrates, carotenoids, and polyacetylenes

Coumarin inhibits the growth of carrot (Daucus carota L. cv. Saint Valery) cells in suspension culture.

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We used a carrot (Daucus carota L. cv. Saint Valery) cell suspension culture as a simplified model system to study the effects of the allelochemical compound coumarin (1,2 benzopyrone) on cell growth and utilisation of exogenous nitrate, ammonium and carbohydrates. Exposure to micromolar levels of

A 2.5-kb insert eliminates acid soluble invertase isozyme II transcript in carrot (Daucus carota L.) roots, causing high sucrose accumulation.

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The predominant storage carbohydrates of mature carrot (Daucus carota L.) storage roots typically are the free sugars glucose and fructose. This trait is conditioned by the Rs allele. A naturally occurring recessive mutation, rs/rs, conditions a shift from these reducing sugars to sucrose. RT-PCR

Pectin methylesterases: sequence-structural features and phylogenetic relationships.

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Pectin methylesterases (PMEs) are enzymes produced by bacteria, fungi and higher plants. They belong to the carbohydrate esterase family CE-8. This study deals with comparison of 127 amino acid sequences of this family containing the five characteristic sequence segments: 44_GxYxE, 113_QAVAL,

Reinforced Polyproline II Conformation in a Hydroxyproline-Rich Cell Wall Glycoprotein from Carrot Root.

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The salt-extractable hydroxyproline-rich cell wall glycoprotein from carrot (Daucus carota L.) roots is composed of 35% (w/w) protein, 3% (w/w) galactose, and 62% (w/w) arabinose. The arabinose is attached to hydroxyproline as tetra- and trisaccharides. The circular dichroism of the glycoprotein

Suboptimal temperature favors reserve formation in biennial carrot (Daucus carota) plants.

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In response to suboptimal temperatures, temperate annual plants often increase root:shoot ratios, build-up carbohydrates and display typical morphological and anatomical changes. We know less about the responses of biennials such as carrot. As a model plant, carrot has the additional feature of two

In situ simultaneous analysis of polyacetylenes, carotenoids and polysaccharides in carrot roots.

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This paper presents an approach to simultaneously analyze polyacetylenes, carotenoids, and polysaccharides in carrot (Daucus carota L.) roots by means of Raman spectroscopy. The components were measured in situ in the plant tissue without any preliminary sample preparation. The analysis is based on

Purification of GP57, and auxin-regulated extracellular glycoprotein of carrots, and its immunocytochemical localization in dermal tissues.

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A glycoprotein (GP57) was purified by ion-exchange and hydroxylapatite column chromatography from the 70%-ethanol precipitate of culture medium of non-embryogenic carrot cells (Daucus carota L.) grown with 2,4-dichlorophen-oxyacetic acid (2,4-D). Its apparent molecular mass (M r) was estimated to be

Investigations on the growth and metabolism of cultured explants of Daucus carota : II. Effects of iron, molybdenum and manganese on metabolism.

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Knowing that the element Fe essentially triggers the action of the coconut milk stimulus for the growth of carrot explants and thereafter interacts with added Mo and Mn, the effects of these trace elements on growth (fresh and dry weight) and metabolism of both nitrogenous and nitrogen-free

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