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Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol.

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A gene encoding a synthetic truncated Candida antarctica lipase B (CALB) was generated via automated PCR and expressed in Saccharomyces cerevisiae. Western blot analysis detected five truncated CALB variants, suggesting multiple translation starts from the six in-frame ATG codons. The longest open

Production of Candida antarctica lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in an ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production.

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A synthetic Candida antarctica lipase B (CALB) gene open reading frame (ORF) for expression in yeast was constructed, and the lycotoxin-1 (Lyt-1) C3 variant gene ORF, potentially to improve the availability of the active enzyme at the surface of the yeast cell, was added in frame with the CALB ORF

Tissue level distribution of toxic and essential elements during the germination stage of corn seeds (Zea mays, L.) using LA-ICP-MS.

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Both essential and toxic metal contaminants impact agricultural crops by bioaccumulation in plants. The goal of this study was to evaluate the tissue-level spatial distribution of metal(loids) in corn seeds (Zea mays, L.) from contaminated corn fields near the Xikuangshan (XKS) antimony mine in

Evaluation of Metaxylem Vessel Histogenesis and the Occurrence of Vessel Collapse during Early Development in Primary Roots of Zea mays ssp. mexicana: A Result of Premature Programmed Cell Death?

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Root apical meristem histological organization in Zea mays has been carefully studied previously. Classical histology describes its system as having a "closed organization" and a development of xylem that conforms to predictable rules. Among the first cell types to begin

Purification and characterization of a glutathione S-transferase from benoxacor-treated maize (Zea mays).

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A glutathione S-transferase (GST) isozyme from maize (Zea mays Pioneer hybrid 3906) treated with the dichloroacetamide herbicide safener benoxacor (CGA-154281) was purified to homogeneity and partially characterized. The enzyme, assayed with metolachlor as a substrate, was purified approximately

Risk assessment of applicators to chlorpyrifos through dermal contact and inhalation at different maize plant heights in China.

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Dermal and respiratory exposure assessments and risk assessment for applicators were performed with 48% chlorpyrifos EC. The chlorpyrifos was applied with lever-operated knapsacks under three spraying environments, namely, average maize field heights of 62 cm (A1), 108 cm (A2), and 212 cm (A3). The

Forestomach neoplasms in Fischer F344/N rats and B6C3F1 mice exposed to diglycidyl resorcinol ether--an epoxy resin.

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Repeated dose (14 days), subchronic (13 wk) and chronic (2 yr) studies were carried out in succession to evaluate the toxic and carcinogenic effects of diglycidyl resorcinol ether (DGRE), a liquid spray epoxy resin, in F344/N rats and B6C3F1 mice. DGRE in corn oil was administered by gavage for 14

Early local differentiation of the cell wall matrix defines the contact sites in lobed mesophyll cells of Zea mays.

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OBJECTIVE The morphogenesis of lobed mesophyll cells (MCs) is highly controlled and coupled with intercellular space formation. Cortical microtubule rings define the number and the position of MC isthmi. This work investigated early events of MC morphogenesis, especially the mechanism defining the

NTP Toxicology and Carcinogenesis Studies of Chlorobenzene (CAS No. 108-90-7) in F344/N Rats and B6C3F1 Mice (Gavage Studies).

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Chlorobenzene is a colorless, volatile liquid under standard environmental conditions (vapor pressure=11.8 mm Hg at 25 degrees C, 760 mm Hg). It is used primarily as a solvent (e.g. resins, dyes, pesticides, and perfumes), a degreasing agent, and a chemical intermediate, particularly in the

Role of copeptin as a novel biomarker of bisphenol A toxic effects on cardiac tissues: biochemical, histological, immunohistological, and genotoxic study.

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Copeptin is a precursor for arginine vasopressin which is usually elevated in acute stress and cardiac emergencies. Bisphenol A (BPA) is an ideal plasticizing factor used in manufacturing of plastics and epoxy resins. To evaluate the cardio toxicity of bisphenol A and to assess copeptin as a cardio

Phloem unloading following reactivation in predarkened mature maize leaves.

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Mature leaf blades of 48-h predarkened maize plants (Zea mays L. cv. Prior) were excised, and treated apically as the source (light, normal air) and basally as the sink (light or dark, air without CO2). After providing the source portion with (14)CO2, the sink portions were harvested after 2, 7 or

NTP Toxicology and Carcinogenesis Studies of Dichlorvos (CAS No. 62-73-7) in F344/N Rats and B6C3F1 Mice (Gavage Studies).

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Toxicology and carcinogenesis studies of dichlorvos (99% pure), a contact and stomach poison for control of insects and parasites, were conducted by administering dichlorvos in corn oil by gavage to groups of F344/N rats and B6C3F1 mice of each sex for 13 weeks or 2 years. Previous feed studies were

Developmental toxicity by exposure to bisphenol A diglycidyl ether during gestation and lactation period in Sprague-Dawley male rats.

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OBJECTIVE Bisphenol A diglycidyl ether (BADGE) is the major component in commercial liquid epoxy resins, which are manufactured by co-reacting bisphenol A with epichlorohydrin. This study was performed to show the developmental effects of prenatal and postnatal exposures to BADGE in male rat

Pharmacodynamics of benzyl chloride in rats.

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In today's world of high industrialization, toxicity and pollution have become common terms of references. Both laymen and experts are becoming increasingly concerned about various health hazards created by occupational and industrial wastes dumped in and around public places. Benzyl chloride (BCl)

Nutritive value of false yam (Icacina oliviformis) tuber meal for broiler chickens.

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False yam (Icacina oliviformis syn. Icacina senegalensis) is a drought-resistant plant in West and Central Africa that produces a large tuber with a high starch content, but it contains antinutritive factors (terpenes). The objective was to evaluate its feed value for broilers when the tuber was

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