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Disposable electrochemical detection of breast cancer tumour marker CA 15-3 using poly(Toluidine Blue) as imprinted polymer receptor.

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In this work, electrically-conducting poly(Toludine Blue) was employed for the first time as synthetic receptor film, prepared by Molecular Imprinting strategies and using electrochemical methods, for the specific screening of breast cancer biomarker Carbohydrate Antigen 15-3 (CA 15-3). The protein

Construction of label-free electrochemical immunosensor on mesoporous carbon nanospheres for breast cancer susceptibility gene.

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In this contribution, mesoporous carbon nanospheres (MCN) were used to fabricate a label-free electrochemical immunosensor for breast cancer susceptibility gene (BRCAl). The detection platform was constructed by conjugation of anti-BRCA1 on glassy carbon electrodes which were modified by mesoporous

Skin test reactivity in patients suffering from lung and breast cancer.

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Mast cells and histamine-mediated reactions may be altered in patients with cancer. In an attempt to characterize the possible skin defects in patients with cancer, we tested 22 patients suffering from lung cancers, 30 from breast cancers, and 30 age-matched normal individuals, using several

[Case of breast cancer with cartilage-like structure].

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A case of breast cancer with cartilage-like structure is presented. The stroma, resembling cartilagenous martrix upon hematoxylin and eosin staining, showed metachromasia upon toluidine blue staining. However, predigestion with hyaluronidase or chondroitinase ABC revealed no change in toluidine blue

Enamel matrix derivative is a potent inhibitor of breast cancer cell attachment to bone.

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This study examined whether enamel matrix derivative (EMD) inhibits the adhesion of cancer cells to bone. A typical breast cancer cell line, MCF-7, was used. Conditioned human osteosarcoma cell (Saos-2) medium was used as extracellular bone matrix (ECBM) to measure cell attachment. MCF-7 cells were

Mammosphere-forming cells from breast cancer cell lines as a tool for the identification of CSC-like- and early progenitor-targeting drugs.

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Here we show that a subpopulation of MCF-7 breast cancer cells which stains pale to Toluidine Blue (Light Cells- LCs), is endowed with features of CSCs. LCs give rise to self-renewing mammospheres and express typical CSC markers; moreover this subpopulation is chemoresistant and highly tumorigenic

Tissue remodelling in breast cancer: human mast cell tryptase as an initiator of myofibroblast differentiation.

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OBJECTIVE Cancerogenesis is characterized by increase of differentiated myofibroblasts. Mast cells (MCs) exert powerful effects on fibroblasts through a variety of mediators. We investigated α-smooth-muscle actin (α-SMA(+) ) and CD34(+) fibroblasts, density of toluidine blue-stained (MCs-TB) and

Tobacco smoke carcinogens and breast cancer.

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Cigarette smoking is an established cause of a variety of cancer types, but its role in breast cancer etiology is not clear. In this report, the potential role of cigarette smoke carcinogens as causes of human breast cancer is evaluated. Of over 60 known carcinogens in tobacco smoke, several are

Modifying the osteoblastic niche with zoledronic acid in vivo-potential implications for breast cancer bone metastasis.

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BACKGROUND Bone metastasis is the most common complication of advanced breast cancer. The associated cancer-induced bone disease is treated with bone-sparing agents like zoledronic acid. Clinical trials have shown that zoledronic acid also reduces breast cancer recurrence in bone; potentially by

Tanshinone IIA inhibits breast cancer stem cells growth in vitro and in vivo through attenuation of IL-6/STAT3/NF-kB signaling pathways.

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Cancer stem cells (CSCs) are maintained by inflammatory cytokines and signaling pathways. Tanshinone IIA (Tan-IIA) possesses anti-cancer and anti-inflammatory activities. The purpose of this study is to confirm the growth inhibition effect of Tan-IIA on human breast CSCs growth in vitro and in vivo

Application of surface enhanced Raman spectroscopy as a diagnostic system for hypersialylated metastatic cancers.

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Early diagnosis of metastatic cancers could greatly limit the number of cancer-associated deaths. Aberrant surface expression of sialic acid (hypersialylation) on tumors correlating with metastatic incidence and its involvement in tumorigenesis and progression is widely reported; hence detection of

[Experimental study on brachial plexus injury induced by radiation in rats].

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OBJECTIVE To explore the changes of morphology and ventricornual motor neurons in SD rats' ventral horn of spinal cord after radiated as the therapy protocol for breast cancer, to discover the rule of radiation-induced injury of brachial plexus, and also if there exits the reversible conversion in

Activation of mast cells within a tumor of angiosarcoma: ultrastructural study of five cases.

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The accumulation of mast cells around tumors is a well-recognized phenomenon in a number of malignancies, including basal cell carcinoma, melanoma, and breast cancer. However, little information exists regarding mast cells within tumor nests. To clarify the role of mast cells infiltrating in skin

NQO1-activated phenothiazinium redox cyclers for the targeted bioreductive induction of cancer cell apoptosis.

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Altered redox signaling and regulation in cancer cells represent a chemical vulnerability that can be targeted by selective chemotherapeutic intervention. Here, we demonstrate that 3,7-diaminophenothiazinium-based redox cyclers (PRC) induce selective cancer cell apoptosis by NAD(P)H:quinone

Detection of monocyclic aromatic amines, possible mammary carcinogens, in human milk.

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Environmental chemicals may play a role in the etiology of human breast cancer. Aromatic amines, industrial chemicals found as environmental pollutants, have been identified as rat mammary carcinogens. We have detected the monocyclic aromatic amines, aniline, o-toluidine, and N-methylaniline at

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