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7 rezultatus
Improved vanillin production in baker's yeast through in silico design.
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BACKGROUND
Vanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods of the vanilla orchid Vanilla planifolia. Currently vanillin is mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous vanillin production from glucose has
De novo biosynthesis of vanillin in fission yeast (Schizosaccharomyces pombe) and baker's yeast (Saccharomyces cerevisiae).
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Vanillin is one of the world's most important flavor compounds, with a global market of 180 million dollars. Natural vanillin is derived from the cured seed pods of the vanilla orchid (Vanilla planifolia), but most of the world's vanillin is synthesized from petrochemicals or wood pulp lignins. We
Establishment of a novel method for the identification of fertilization defective mutants in Arabidopsis thaliana.
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Plant reproduction is an extremely important phenomenon, as it is strongly associated with plant genetics and early development. Additionally, foundations of the reproductive system have direct implications on plant breeding and agriculture. Investigation of the functions of male and female
Phytotoxic effects of 21 plant secondary metabolites on Arabidopsis thaliana germination and root growth.
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This study investigated potential phytotoxic effects on germination and root growth of 21 plant secondary metabolites (sinapinic, syringic, vanillic, ferulic, p-coumaric, chlorogenic, gallic, gentisic, protocatechuic, p-hydroxybenzoic, and trans-cinnamic acids, and eucalyptol, quercetin, vanillin,
Arabidopsis Chy1 null mutants are deficient in benzoic acid-containing glucosinolates in the seeds.
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The specific set of reactions that lead to the synthesis of benzoic acid in plants is still unclear, and even the subcellular compartment in which these reactions occur is unknown. Biosynthesis of both vegetative tissues and seeds of Arabidopsis thaliana contain a class of defense compounds termed
Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis.
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The testa of higher plant seeds protects the embryo against adverse environmental conditions. Its role is assumed mainly by controlling germination through dormancy imposition and by limiting the detrimental activity of physical and biological agents during seed storage. To analyze the function of
Heterologous expression of the plant coumarate: CoA ligase in Lactococcus lactis.
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OBJECTIVE
To demonstrate the expression of coumarate : CoA ligase of Arabidopsis thaliana in Lactococcus lactis as a first step of cloning the vanillin pathway.
RESULTS
The 4CL gene was amplified from a cDNA library of A. thaliana by PCR and subcloned into a multicopy lactococcal vector where the
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