Aberrant crypt foci (ACF) are clusters of abnormal tube-like glands in the lining of the colon and rectum. Aberrant crypt foci form before colorectal polyps and are one of the earliest changes seen in the colon that may lead to cancer. ACF are, as opposed to normal epithelial cells, apoptosis resistant. When looking for aberrant crypt foci with microscopy, methylene blue is used as a staining agent.
The resulting figure is fairly easy to detect under the microscope at low magnification (x40).Chemically ...
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