[Immunomodulatory activity and anti-tumor activity of Oldenlandia diffusa in vitro].

OBJECTIVE

To study the effect of Oldenlandia diffusa (OD) on lymphocytes and tumor cells in vitro.

METHODS

Effects of OD extract (ODE) on proliferation of spleen cells of mice and the phagocytosis of monocytes to tumor cells using 3H incorporation were analysed, and effect on specific activity of natural killer (NK) cells to human and mice's tumor cells was determined using free 51 Cr experiments, effects on B cells' antibody production and cytokine of monocyte production were investigated by ELISA and biological method, the chemical composition of OD was analyzed by chromatography, protease digestion and sugar decomposition (NaIO4) test.

RESULTS

ODE could vigorously promote the proliferative activity of spleen cells in mice, specific lethal activity of human and mice's NK cell to tumor cells, B cells' antibody production, monocytes' cytokine production and its phagocytosis to remove the tumor cells. By means of chromatography, protease E digestion and NaIO4 treatment, the result of chemical composition analysis showed that the component of OD was 90 kDa glycoprotein.

CONCLUSIONS

ODE has immuno-modulating activity and anti-tumor activity in vitro through stimulating the immune system to kill or engulf tumor cells, which could be used clinically for immune function modulation and to treat tumor and other diseases.