Metabolome analysis of rice leaves to obtain low-oxalate strain from ion beam-mutagenised population

Introduction: Rice leaves and stems, which can be used as rice straw for livestock feed, accumulate soluble oxalate. The oxalate content often reaches 5% of the dry weight leaves. Excess uptake of oxalate-rich plants causes mineral deficiencies in vertebrates, so it is important to reduce the oxalate content in rice leaves to produce high-quality rice straw. However, the mechanism of oxalate accumulation in rice has remained unknown.

Objectives: To understand metabolic networks relating oxalate accumulation in rice.

Methods: In this study, we performed metabolome analysis of rice M₂ population generated by ion-beam irradiation using CE-MS.

Results: The result showed wide variation of oxalate contents in M₂ plants compared with those of control plants. Multivariate analyses of metabolome dataset revealed that oxalate accumulation was strongly related with anionic compounds such as 2OG and succinate. For low-oxalate plants, four patterns of metabolic alterations affected oxalate contents in the M₂ leaves were observed. In M₃ plants, we found putative low-oxalate line obtained from low-oxalate M₂ mutant.

Conclusions: These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.These findings would lead to produce the low-oxalate rice and to understand the oxalate synthesis in plants.

Keywords: CE-MS; Carbon ion beam; Metabolic alterations; Oryza sativa; Oxalate.