The role of cyanide-resistant respiration in Solanum tuberosum L. against high light stress.
Atslēgvārdi
Abstrakts
The cyanide-resistant respiration that occurs in potato mitochondria is one of the important pathways for energy dissipation. It can be activated by glare. However, it is unclear what roles cyanide-resistant respiration play in the high-light stress of potatoes. This study designed a CRISPR vector for the functional gene StAOX of the potato cyanide-resistant respiratory. Agrobacterium tumefaciens GV3101 was transformed into potato. H2 O2 level, MDA content, antioxidant activity and Cyanide-resistant respiratory capacity of potato leaves under high light stress were determined. Photosynthetic efficiency and chlorophyll content were determined. In addition, the operation of the malate-oxaloacetate shuttle route and the transcription level of photorespiration related enzymes were also examined. The results showed that two base substitutions occurred at the sequencing target site on the leaves of a transformed potato. Accumulation of active oxygen and the increase of membrane lipid peroxidation were detected in the transformed potato leaves. Lower photosynthetic efficiency was observed. The transcription level of the malate-oxaloacetate shuttle route and photorespiration-related enzymes were also significantly increased. These results indicated that cyanide-resistant respiration is an important physiological process in the potato against high light stress. It also suggested that the plant cyanide-resistant respiration is closely related to photosynthesis. It implies the unexplored importance of plant cyanide-resistant respiration to plant photosynthesis, energy conversion, and carbon skeleton formation.