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brefeldin a/kukurūza

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F-actin-dependent endocytosis of cell wall pectins in meristematic root cells. Insights from brefeldin A-induced compartments.

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Ielogoties Reģistrēties
Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response to BFA treatment is a unique feature of some, but not all, cells. Here, we have

Recycling of Solanum sucrose transporters expressed in yeast, tobacco, and in mature phloem sieve elements.

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Ielogoties Reģistrēties
The plant sucrose transporter SUT1 (from Solanum tuberosum, S. lycopersicum, or Zea mays) exhibits redox-dependent dimerization and targeting if heterologously expressed in S. cerevisiae (Krügel et al., 2008). It was also shown that SUT1 is present in motile vesicles when expressed in tobacco cells

KDEL-Containing Auxin-Binding Protein Is Secreted to the Plasma Membrane and Cell Wall.

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Ielogoties Reģistrēties
The auxin-binding protein ABP1 has been postulated to mediate auxin-induced cellular changes associated with cell expansion. This protein contains the endoplasmic reticulum (ER) retention signal, the tetrapeptide lysine-aspartic acid-glutamic acid-leucine (KDEL), at its carboxy terminus, consistent

A role for actin-driven secretion in auxin-induced growth.

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Ielogoties Reģistrēties
In epidermal cells of Zea mays coleoptiles, actin microfilaments are organized in fine strands during cell elongation, but are bundled in response to signals that inhibit growth. This bundling response is accompanied by an increased membrane association of extracted actin. Brefeldin A, an inhibitor

Short-term boron deprivation inhibits endocytosis of cell wall pectins in meristematic cells of maize and wheat root apices.

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Ielogoties Reģistrēties
By using immunofluorescence microscopy, we observed rapidly altered distribution patterns of cell wall pectins in meristematic cells of maize (Zea mays) and wheat (Triticum aestivum) root apices. This response was shown for homogalacturonan pectins characterized by a low level (up to 40%) of

Distribution of expansins in graviresponding maize roots.

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Ielogoties Reģistrēties
To test if expansins, wall loosening proteins that disrupt binding between microfibrils and cell wall matrix, participate in the differential elongation of graviresponding roots, Zea mays L. cv. Merit roots were gravistimulated and used for immunolocalization with anti-expansin. Western blots showed

Sucrose synthase is associated with the cell wall of tobacco pollen tubes.

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Ielogoties Reģistrēties
Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for
SE-WAP41, a salt-extractable 41-kD wall-associated protein that is associated with walls of etiolated maize (Zea mays) seedlings and is recognized by an antiserum previously reported to label plasmodesmata and the Golgi, was cloned, sequenced, and found to be a class 1 reversibly glycosylated
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