glioma/triacylglycerol

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13C-labelling studies indicate compartmentalized synthesis of triacylglycerols in C6 rat glioma cells.

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Ielogoties Reģistrēties

NMR-visible mobile lipid (ML) signals have been detected in (1)H-NMR spectra of tissues in vivo, ex vivo and in vitro, and have been shown to change in apparent intensity in association with pathology (necrosis in brain tumours) and normal processes (cell differentiation, cell growth arrest and

Involvement of triacylglycerol in the metabolism of fatty acids by cultured neuroblastoma and glioma cells.

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Ielogoties Reģistrēties

The metabolism (chain elongation, desaturation, and incorporation into complex lipids) of thirteen different radiolabeled fatty acids and acetate was examined in N1E-115 neuroblastoma and C-6 glioma cell lines in culture. During 6-hr incubations, all fatty acids were extensively (14-80%) esterified

Metabolite changes in BT4C rat gliomas undergoing ganciclovir-thymidine kinase gene therapy-induced programmed cell death as studied by 1H NMR spectroscopy in vivo, ex vivo, and in vitro.

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Ielogoties Reģistrēties

Programmed cell death was induced by HSV-tk gene therapy in rat BT4C glioma cells, and metabolite changes associated with cell damage were monitored in vivo by 1H NMR spectroscopy and ex vivo by high resolution magic angle spinning (HRMAS) 1H NMR, and in vitro in perchloric acid extracts of tumors.

Interaction of (n-3) and (n-6) fatty acids in desaturation and chain elongation of essential fatty acids in cultured glioma cells.

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Ielogoties Reģistrēties

Recent research in various biological systems has revived interest in interactions between the (n-6) and (n-3) essential fatty acids. We have utilized cultured glioma cells to show that linolenic acid, 18:3(n-3), is rapidly desaturated and chain elongated; 20:5(n-3) is the major product and

Thapsigargin selectively stimulates synthesis of phosphatidylglycerol in N1E-115 neuroblastoma cells and phosphatidylinositol in C6 glioma cells.

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Ielogoties Reģistrēties

Phospholipid metabolism was studied in N1E-115 neuroblastoma and C6 glioma cells exposed to thapsigargin, a selective inhibitor of endoplasmic reticulum Ca(2+)-ATPase that raises the cytosolic free Ca2+ concentration [Ca2+]i. Thapsigargin caused only a transient increase of [Ca2+]i (< 1 min) in

Ethanol alters the transfer of arachidonic acid to ethanolamine plasmalogens in C-6 glioma cells.

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Ielogoties Reģistrēties

In this study, the effects of ethanol exposure on uptake and metabolism of arachidonic acid by C-6 glioma cells in culture was examined. Labeled arachidonic acid was effectively taken up by the phospholipids of these cells and radioactivity was initially incorporated into phosphatidylinositols and

Studies of the modulation of essential fatty acid metabolism by fatty acids in cultured neuroblastoma and glioma cells.

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Ielogoties Reģistrēties

In cultured neuroblastoma cells (N1E-115), the metabolism of the essential fatty acid, linoleic acid (18:2 (n-6)), to arachidonic acid (20:4(n-6)) can be altered by other fatty acids in a manner supporting a concerted action of the modulating fatty acid on the desaturation and chain elongation

A possible cellular explanation for the NMR-visible mobile lipid (ML) changes in cultured C6 glioma cells with growth.

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Ielogoties Reģistrēties

The NMR-visible mobile lipid (ML) signals of C6 glioma cells have been monitored at 9.4 and 11.7 T (single pulse and 136 ms echo time) from cell pellets by (1)H NMR spectroscopy. A reproducible behavior with growth has been found. ML signals increase from log phase (4 days of culture) to

Impact of mitochondrial beta-oxidation in fatty acid-mediated inhibition of glioma cell proliferation.

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Ielogoties Reģistrēties

Tetradecylthioacetic acid (TTA), which cannot be beta-oxidized, exerts growth-limiting properties in glioma cells. In order to investigate the importance of modulated lipid metabolism and alterations in mitochondrial properties in this cell death process, we incubated glioma cells both with TTA and

Lipidomic analysis reveals a radiosensitizing role of gamma-linolenic acid in glioma cells.

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Ielogoties Reģistrēties

Previous studies have demonstrated that gamma-linolenic acid (GLA) is effective against glioma cells under both in vitro and in vivo conditions. In the present study we determined how GLA alone or in combination with irradiation alters the fatty acid (FA) and lipid profiles, the lipid droplet (LD)

Investigation of serum proteome alterations in human glioblastoma multiforme.

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Ielogoties Reģistrēties

Glioblastoma multiforme (GBM) or grade IV astrocytoma is the most common and lethal adult malignant brain tumor. The present study was conducted to investigate the alterations in the serum proteome in GBM patients compared to healthy controls. Comparative proteomic analysis was performed employing

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