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nitrate/soja

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Lappuse 1 no 196 rezultātiem

Regulation of GmNRT2 expression and nitrate transport activity in roots of soybean (Glycine max).

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Ielogoties Reģistrēties
A full-length cDNA, GmNRT2, encoding a putative high-affinity nitrate transporter was isolated from a Glycine max (L.) root cDNA library and sequenced. The deduced GmNRT2 protein is 530 amino acids in length and contains 12 putative membrane-spanning domains and a long, hydrophilic C-terminal
Although nitrate is known to inhibit nodulation and N2 fixation in symbiotic legumes, little is known about its effect on the uptake and accumulation of trace elements such as Fe, Zn, Mn and Cu. The aim of this study was to evaluate the effect of 5 mM NO3- supply, either with or without rhizobial
Soybean (Glycine max [L.] Merr.) leaves have been shown to contain three forms of nitrate reductase (NR). Two of the forms, which are present in leaves of wild-type (cv. Williams) plants grown in the absence of NO(3) (-), are termed constitutive and designated c(1)NR and c(2)NR. The third form,
Biological N(2) fixation can fulfil the N demand of legumes but may cost as much as 14% of current photosynthate. This photosynthate (C) sink strength would result in loss of productivity if rates of photosynthesis did not increase to compensate for the costs. We measured rates of leaf
The ATP-dependent proton-pumping activity of soybean (Glycine max L.) root microsomes is predominantly nitrate sensitive and presumably derived from the tonoplast. We used microsomes to characterize anion effects on proton pumping of the tonoplast vesicles using two distinctly different

Isolation and properties of soybean [Glycine max (L.) Merr.] mutants that nodulate in the presence of high nitrate concentrations.

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Ielogoties Reģistrēties
Soybean seeds [Glycine max (L.) Merr. ev. Bragg] were mutagenized with ethyl methanesulfonate. The M(2) progeny (i.e., the first generation after mutagenesis) of these seeds were screened for increased nodulation under high nitrate culture conditions. Fifteen independent nitrate-tolerant symbiotic

Isolation and Initial Characterization of Constitutive Nitrate Reductase-Deficient Mutants NR328 and NR345 of Soybean (Glycine max).

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Ielogoties Reģistrēties
Two nitrate reductase deficient mutants of soybean (Glycine max [L.] Merr. cv Bragg) were isolated from approximately 10,000 M(2) seedlings, using a direct enzymic assay in microtiter plates. Stable inheritance of NR345 and NR328 phenotypes has been demonstrated through to the M(5) generation. Both
NADH:nitrate reductase (EC 1.6.6.1) and NAD(P)H:nitrate reductase (EC 1.6.6.2) were purified from wild-type soybean (Glycine max [L.] Merr., cv Williams) and nr(1)-mutant soybean plants. Purification included Blue Sepharose- and hydroxylapatite-column chromatography using acetone powders from fully

Nitrate Reductase Activity in Soybeans (Glycine max [L.] Merr.): I. Effects of Light and Temperature.

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Ielogoties Reģistrēties
The optimum in vivo nitrate reductase (NR) assay medium for soybean (Glycine max [L.] Merr.) leaves was 50 mm KNO(3), 1% (v/v) 1- propanol, and 100 mm potassium phosphate buffer (pH 7.5).Loss of in vivo NR activity from leaves of soybeans exposed to dark was fastest at 40 C and slowest at 20 C.

Nitrate Reductase Activity in Soybeans (Glycine max [L.] Merr.): II. Energy Limitations.

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Ielogoties Reģistrēties
Growth chamber studies with soybeans (Glycine max [L.] Merr.) were designed to determine the relative limitations of NO(3) (-), NADH, and nitrate reductase (NR) per se on nitrate metabolism as affected by light and temperature. Three NR enzyme assays (+NO(3) (-)in vivo, -NO(3) (-)in vivo, and in

Nitrate Reduction by Roots of Soybean (Glycine max [L.] Merr.) Seedlings.

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Ielogoties Reģistrēties
Studies were conducted with 9 to 12 day-old soybean (Glycine max [L.] Merr. cv. Williams) seedlings to determine the contribution of roots to whole plant NO(3) (-) reduction. Using an in vivo -NO(3) (-) nitrate reductase (NR) assay (no exogenous NO(3) (-) added to incubation medium) developed for

Canopy and Seasonal Profiles of Nitrate Reductase in Soybeans (Glycine max L. Merr.).

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Ielogoties Reģistrēties
Nitrate reductase activity of soybeans (Glycine max L. Merr.) was evaluated in soil plots and outdoor hydroponic gravel culture systems throughout the growing season. Nitrate reductase profiles within the plant canopy were also established. Mean activity per gram fresh weight per hour of the entire

Efficiency of Nitrogen Assimilation by N(2)-Fixing and Nitrate-Grown Soybean Plants (Glycine max [L.] Merr.).

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Ielogoties Reģistrēties
Nodulated and non-nodulated (not inoculated) soybeans (Glycine max [L.] Merr. cv Wells) were grown in controlled environments with N(2) or nonlimiting levels of NO(3) (-), respectively, serving as sole source of nitrogen. The efficiency of the N(2)-fixing plants was compared with that of the
We studied the influence of exogenic ammonium on the functional activity and ultrastructural organization of cells of the mixotrophic soybean callus (Glycine max L.). Ammonium available in the nutrient medium increased the chlorophyll content, accelerating the rate of photosynthetic O2 evolution per

Influence of Temperature on Nitrate Metabolism and Leaf Expansion in Soybean (Glycine max L. Merr.) Seedlings.

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Ielogoties Reģistrēties
The effect of various day temperatures on NADH-nitrate reductase, NADH- and NADPH-glutamate dehydrogenases, nitrate, protein and leaf area, measured at intervals during the ontogeny of the first trifoliolate soybean leaf, was determined. At 32.5 C and 25 C, nitrate concentration, nitrate reductase,
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