ANTIOXIDANT, ANTI-INFLAMMATORY ACTIVITY OF GEORGIAN LEGUMINOUS CROPS CULTURES.

The aim of the study was to identify the common in Georgia leguminous crops culture with pronounced antioxidant, anti-inflammatory activity. The primary evaluation of the antiinflammatory effects of beans was performed on the experimental models of MDCK and Jurkat cells model systems. Extracts of various varieties of legumes (Beans "Kidney", Meadow beans, Beans Shulavera, Batumian beans, Beans "Udelebi", green peas, peas Shulavera, lentils Lens Culinaris, Soy) were added to the intact or incubated under oxidative stress conditions Jurkat and MDCK cells. Cells' vitality was determined by MTT test. On the basis of analysis of the obtained results, we concluded that: - Meadow beans extract (low doses) revealed cytoprotective effect on the intact and incubated under oxidative stress conditions immune (Jurkat) and epithelial (MDCK) cells. High antioxidant, cytoprotective activity of this extract correlates with high polyphenols content in it. - The extract of Shulavera beans did affect the intact Jurkat and MDCK cells, but showed pronounced cytoprotective activity on these cells incubated under the oxidative stress conditions. High antioxidant, cytoprotective activity of this extract correlates with high content of polyphenols in it. - Low dose of lentils Lens Culinaris extracts revealed cytoprotective activity on the incubated under oxidative stress conditions MDCK cells, but was inactive in case of intact MDCK and incubated in different conditions immune Jurkat cells. The selective antioxidant activity of this extract is related with its other constituent components, but not polyphenols. - Despite high polyphenols content and high antioxidant activity in vivo, Batumian beans revealed moderate cytoprotective activity on intact and incubated under oxidative stress conditions Jurkat cells, suppressive activity on the intact MDCK cells and was inactive in relation to the incubated under oxidative stress conditions MDCK cells. Based on these findings, we can identify extracts with selective protective, anti-inflammatory (Meadow beans, Shulavera beans extracts), cytoprotective (lentil Lens Culinaris extract) and immunomodulatory (Butumian bean extract) properties. Further studies are needed to identify and verify the mechanisms of activity of these Extracts in order to develop effective selective dietary supplements.