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hyoscyamus/nicotiana tabacum
Врската е зачувана во таблата со исечоци
Страница 1 од 21 резултати
Hyoscyamus muticus + Nicotiana tabacum fusion hybrids selected via auxotroph complementation.
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Protoplasts of the nicotinamide-deficient Hyoscyamus muticus cell line nic(-) IVH2 and of the nitrate reductase-deficient Nicotiana tabacum cell line NR(-) cnx 68 were induced to fuse. Selection for putative interspecific hybrid clones was via auxotroph complementation. Controls included tests for
Self-fertile cybrids Nicotiana tabacum (+ Hyoscyamus aureus) with a nucleo-plastome incompatibility.
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Cytoplasmic hybrids (cybrids) in a novel inter-generic combination, Nicotiana tabacum (+ Hyoscyamus aureus), were generated by fusion of protoplasts from a plastome tobacco albino mutant (line R100a1) and gamma-irradiated green protoplasts of H. aureus. Cybrids possessed a plastome of H. aureus and
New CMS-associated phenotypes in cybrids Nicotiana tabacum L. (+Hyoscyamus niger L.).
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Morphological characteristics were studied in cytoplasmic male sterile (CMS) cybrids possessing the tobacco nuclear genome, Hyoscyamus niger plastome and recombinant mitochondria. After backcrosses with tobacco, new flower modifications were found, including: conversions of stamens into branched
Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo-cytoplasmic incompatibility.
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The genetic basis of multiple phenotypic alterations was studied in cell-engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of
Promotion of nicotine biosynthesis in transgenic tobacco by overexpressing allene oxide cyclase from Hyoscyamus niger.
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Plant secondary metabolites are a wide variety of low-molecular weight compounds whose productions are often enhanced in response to both biotic and abiotic stresses. Many of the responses are mediated by a class of hormones, named as jasmonates. In jasmonate biosynthetic pathway of plants, allene
Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent.
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The cDNA from Nicotiana tabacum encoding Putrescine N-methyltransferase (PMT), which catalyzes the first committed step in the biosynthesis of tropane alkaloids, has been introduced into the genome of a scopolamine-producing Hyoscyamus niger mediated by the disarmed Agrobacterium tumefaciens strain
In vitro hybridization by sexual methods and by fusion of somatic protoplasts : Experiments with Nicotiana tabacum x Petunia hybrida, N. tabacum x Hyoscyamus niger, H. niger x P. hybrida, Melandrium album x P. hybrida.
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1. No hybrid plants of Nicotiana tabacum + Petunia hybrida were regenerated from calluses of fusion experiments with mesophyll protoplasts of N. tabacum s, s (2) and v and of P. hybrida mu 1 (2). 2. After in vitro pollination of ovules of N. tabacum with pollen of P. hybrida, filamentous proembryos
Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase.
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Hyoscyamine-6beta-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S
Species-specific repetitive DNA used to identify interspecific somatic hybrids.
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Plasmid DNA clones containing repetitive DNA sequences were isolated from Hyoscyamus muticus and Nicotiana tabacum. Non cross-hybridizing probes from each species were used in a simple hybridization test with DNA isolated from presumptive somatic hybrids. This allowed unequivocal identification of
Identifying functional domains within terpene cyclases using a domain-swapping strategy.
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Cyclic terpenes and terpenoids are found throughout nature. They comprise an especially important class of compounds from plants that mediate plant- environment interactions, and they serve as pharmaceutical agents with antimicrobial and anti-tumor activities. Molecular comparisons of several
X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products.
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Complementation of two metabolic deficiences - nitrate reductase and tryptophan synthase - was used to select for somatic fusion hybrids between tobacco (Nicotiana tabacum) and henbane (Hyoscyamus muticus) with prior X-irradiation of one partner. Using species specific, radioactively labelled DNA
Reconstitution of plant nitrate reductase by Escherichia coli extracts and the molecular cloning of the chlA gene of Escherichia coli K12.
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Extracts from Escherichia coli, wild type and chlB, chlC, chlD, chlE, and chlG, but not chlA mutants, were able to reconstitute the nitrate reductase activity in Nicotiana tabacum cnx68 and Hyoscyamus muticus MA-2 mutant extracts. Because cnx68 and MA-2 lack the molybdenum cofactor required for
Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.
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Two novel techniques improve division and colony formation from protoplasts: 1) Plating in agarose stimulates colony formation of protoplasts from a wide range of species. Protoplasts from Nicotiana tabacum developed to colonies from lower initial population densities in agarose than in agar or
Quantitative exploration of the catalytic landscape separating divergent plant sesquiterpene synthases.
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Throughout molecular evolution, organisms create assorted chemicals in response to varying ecological niches. Catalytic landscapes underlie metabolic evolution, wherein mutational steps alter the biosynthetic properties of enzymes. Here we report the first systematic quantitative characterization of
Biosynthetic potential of sesquiterpene synthases: product profiles of Egyptian Henbane premnaspirodiene synthase and related mutants.
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The plant terpene synthase (TPS) family is responsible for the biosynthesis of a variety of terpenoid natural products possessing diverse biological functions. TPSs catalyze the ionization and, most commonly, rearrangement and cyclization of prenyl diphosphate substrates, forming linear and cyclic
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