A method of detection of neoplastic tissue comprising, providing a first solution, the first solution comprising cytosol malate dehydrogenase from a mammalian test tissue, determining a first rate or a first function of the first rate at which the first solution catalyzes the reaction of oxaloacetate to malate, comparing the first rate or first function of the first rate with a known standard normal rate or function of the normal rate at which a normal solution of cytosol malate dehydrogenase catalzes the reaction of oxaloacetate to malate, whereby the first rate or the first function of the first rate being substantially higher than the known standard normal rate or function of the normal rate indicated that the mammalian test tissue is neoplastic.

Malate dehydrogenase method

The invention provides compounds and methods of their use in the detection of apoptosis and necrosis both in vitro and in vivo. Also provided are compounds and methods of their use in selective delivery of agents to cells undergoing apoptosis or necrosis. The compounds and methods are based on conjugates formed with a dehydrogenase such as lactate dehydrogenase, alcohol dehydrogenase, aldehyde dehydrogenase, and malate dehydrogenase. The compounds and methods are useful in the diagnosis and treatment of conditions characterized by apoptosis, including cancer, cardiac disease, neurologic disease including stroke, and autoimmunity. The compounds and methods offer distinct advantages over corresponding compounds and methods based on Annexin V. Also provided are methods for screening for compounds that modulate, i.e., inhibit or promote, apoptosis.

malate dehydrogenase/рак

Malate dehydrogenase method

Compositions for detecting cell death and methods of use thereof

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