Conversion of human fibroblasts to tissue macrophages by the Snyder-Theilen sarcoma virus (ST:FeSV): tumouricidal effects on K-562 and Daudi tumour cell lines in monolayers and in agar suspensions.
Түлхүүр үгс
Хураангуй
We have previously demonstrated the conversion of human fibroblasts (HF) to tissue macrophages by transduction with the Snyder-Theilen feline sarcoma virus (ST:FeSV) [1-3]. The ST:FeSV-induced TM have been characterized both phenotypically and functionally, including their tumouricidal potential against colon adenocarcinoma (LS 180) cells. The present results show that ST:FeSV-induced TM produced significant lysis of K-562 tumour cells, but essentially no lysis of the Daudi tumour cells. Lysis of K-562 tumour cells by the ST:FeSV-induced TM was considerably more effective in 1% than in 10% FCS. Addition of TNF-alpha caused only a slight increase in the extent of lysis of K-562 tumour cells by the ST:FeSV-induced TM. Coincubation of the ST:FeSV-induced TM with K-562 cells in agar medium resulted in the inhibition of tumour cell proliferation. The results indicate that ST:FeSV-induced TM are potent oncocytotoxic agents of K-562 tumour cells, but are considerably less effective against the Daudi tumour cells.