Oxidative damage and hepatotoxicity associated with deltamethrin in rats: The protective effects of Amaranthus spinosus seed extract.

OBJECTIVE

The liver plays a major role in detoxification and metabolism of toxic compound and xenobiotics like pesticides. Thereby, any type of modification in its function causes hepatotoxicity. So, the aim of this study was designed to assess the antioxidant and hepatoprotective effects of methanol extract Amaranthus spinosus seed (ASS) on liver injury induced by deltamethrin (DLM), a synthetic pyrethroid pesticide.

METHODS

ASS was subjected to different phytochemical analyses including total phenolic and flavonoid content. In vitro, antioxidant activities was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide scavenging assays. In vivo hepatoprotective effect of A. spinosus seeds against DLM induced liver injury in rats was tested in plasma and liver tissue.

RESULTS

ASS reverses hepatotoxicity in DLM-intoxicated rats: serum liver biomarkers (alkaline phosphatase, lactate dehydrogenase and aminotransferases), hepatic levels of lipid peroxidation (MDA), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and glutathione peroxidase (GPx) were restored to normal levels. The protective effects of ASS was confirmed by histological studies.

CONCLUSIONS

The hepatoprotective potential of ASS could be explained by its high phenolic content, antioxidant properties and phytochemical contents.