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Genes and Genomics 2020-Jul

Expression and evolution of the phospholipase C gene family in Brachypodium distachyon

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Xianguo Wang
Yang Liu
Zheng Li
Xiang Gao
Jian Dong
Mingming Yang

Sleutelwoorden

Abstract

Background: Phospholipase C (PLC) is an enzyme that hydrolyzes phospholipids and plays an important role in plant growth and development. The Brachypodium distachyon is a model plant of Gramineae, but the research on PLC gene family of Brachypodium has not been reported.

Objective: This study was performed to identify the PLC family gene in Brachypodium and to determine the expression profiles of PLCs under the abiotic stress.

Methods: Complete genome sequences and transcriptomes of Brachypodium were downloaded from the PLAZA. The hidden Markov model-based profile of the conserved PLC domain was submitted as a query to identify all potential PLC domain sequences with HMMER software. Expression profiles of BdPLCs were obtained based on the qRT-PCR analysis.

Results: There were 8 PLC genes in Brachypodium (BdPI-PLCs 1-4 and BdNPCs 1-4). All members of BdPI-PLC had three conserved domains of X, Y, and C2, and no EF-hand was found. All BdNPCs contained a phosphatase domain. BdPI-PLC genes were distributed on Chr1, Chr2 and Chr4, with different types and numbers of cis-regulatory elements in their respective gene promoters. Phylogenetic analysis showed that the genetic relationship between Brachypodium and rice was closer than Arabidopsis. The expression patterns of BdPI-PLC gene under abiotic stresses (drought, low temperature, high temperature and salt stress) were up-regulated, indicated their important roles in response to low temperature, high temperature, drought and salt stresses.

Conclusions: This study provides comprehensive information for the study of Brachypodium PLC gene family and lays a foundation for further research on the molecular mechanism of Brachypodium stress adaptation.

Keywords: Abiotic stress; Brachypodium distachyon; Expression; Family; Phospholipase C.

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