Lignan compound isolated from n-Hexane extract myristica fragrans Houtt root as antioxidant and antitumor activities against MCF-7 cell lines data

Nutmeg plant (Myristica fragrans Houtt) is known as one of traditional medicine. The nutmeg root has a strong potential in antioxidant and anticancer agents among other nutmeg plant parts. The n-hexane root extract has been carried out by thin-layer chromatography and obtained 8 fractions (labeled as Myristica fragrans Houtt Root: MFHR 1 - 8). Specifically, the MFHR 4 has been purified for several times to obtain a yellow-brown color. Furthermore, lignan compound 6'-methyl-(7‑hydroxy-8-methylbut-9-en)-3,2'-dimethoxybiphenyl-4,5-diol) was identified with chemical formula of C₂₀H₂₄O₅ and analyzed using UV-vis spectroscopy, fourier-transform infrared spectroscopy (FTIR), 1D/2D nuclear magnetic resonance (NMR), and liquid chromatography-mass spectrometry (LC-MS). Based on MTT assay, MFHR demonstrated moderate anticancer activity against MCF-7 cell lines of 51.95 µM, meanwhile, DPPH activity confirmed the strong antioxidant activity with IC50 value of 12.67 ppm.

Keywords: Anticancer; Antioxidant; Lignan; N-hexane extract; Nutmeg root.