Materials and Methods for use in treating cell proliferative disorders related to asparagine metabolism are provided. Cell proliferative disorders include such cancers as forms of leukemia, ovarian cancers, melanomas, renal cancers, breast cancers, brain cancers, and other cancers. Methods include the use of RNA interference targeted at asparagine synthetase to enhance the efficacy of L-asparaginase therapies.

Lorenzi; Philip L.

Weinstein; John N.

Caplen; Natasha J.

The United States of America, as represented by the Secretary, Department of Health and Human Services

Materials and methods directed to asparagine synthetase and asparaginase therapies

The United States of America as represented by the Department of Health and Human Services

The present invention provides a process for synthesizing a glycopeptide having the structure: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7 and R.sub.8 are each independently H, OH, OR.sup.1, NH.sub.2, NHCOR.sup.i, F, CH.sub.2 OH, CH.sub.2 OR.sup.i, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyl or aryl group, where R.sup.i is H, CHO, CO.sub.2 R.sup.ii, a linear or branched chain alkyl, arylalkyl or aryl group, or an oligosaccharide moiety, etc., wherein R.sub.0 is H, a linear or branched chain alkyl, arylalkyl or aryl group; wherein R.sub.10 is a substituted or unsubstituted linear or branched chain acyl, arylacyl or aroyl group; wherein R.sub.11[x] represents X amino acid side-chains, and denotes position from the N-terminus, and R.sub.13[y] represents Y amino acid side-chains, and denotes position from the C-terminus, wherein a, b, c, h, i, j, r, s and t are each independently an integer between about 0 and about 3; wherein m and n are each independently an integer between about 0 and about 5; and wherein x, x'(N), y and y'(N) are each independently an integer between about 0 and,about 25; such glycopeptides being useful as a vaccine for inducing antibodies to human breast cancer cells in an adjuvant therapy therefor.

asparagine/borstkanker

Materials and methods directed to asparagine synthetase and asparaginase therapies

Materials and methods directed to asparagine synthetase and asparaginase therapies

Synthesis of asparagine-linked glycopeptides on a polymeric solid support

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